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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (24): 78-82.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

植物组织双向电泳样品制备方法研究进展

李德军,邓 治,陈春柳,陈守才   

  • 收稿日期:2009-09-07 修回日期:2009-09-11 出版日期:2009-12-20 发布日期:2009-12-20
  • 基金资助:

    国家天然橡胶现代农业产业技术体系建设项目;中国热带农业科学院橡胶研究所基本科研业务费专项资金项目

Research Progress on Protein Extraction from Plant Tissues for Two-dimensional Gel Electrophoresis

  • Received:2009-09-07 Revised:2009-09-11 Online:2009-12-20 Published:2009-12-20

PDF (PC)

1571

摘要：

摘要：双向电泳是目前应用最广泛的蛋白分离技术，该技术的核心是蛋白样品的制备。植物组织中通常含有大量蛋白酶和次生代谢物，这严重干扰蛋白提取、分离和鉴定。从植物组织中提取高质量的蛋白样品用于蛋白组分析充满挑战，本文简要阐述了植物组织蛋白提取的关键点，特别是植物组织中的次生代谢物及去除方案。此外，本文还对三种植物蛋白提取方法、三种方法的优缺点及适用植物组织作了概述。

关键词: 籼粳亚种间杂交水稻, 籼粳亚种间杂交水稻, 单季晚稻, 栽培技术

Abstract:

Abstract: The two-dimensional gel electrophoresis（2-DE）is the most popular techniques for protein separation, and the key technology of 2-DE is the protein extraction. Plant tissues usually contain high levels of proteases and secondary metabolites that severely interfere with protein extraction, separation, and identification. Preparing the high-quality protein samples from plant tissues for proteomic analysis faces a great challenge. This article briefly describes the critical points on protein separation, especially secondary metabolites in plant tissues, and removal strategies. In addition, it also provides an updated overviews of three total protein extraction methods, their advantages and disadvantages and their applications for protein extraction from plant tissues.