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中国农学通报 ›› 2010, Vol. 26 ›› Issue (3): 142-145.

• 林学 园艺 园林 • 上一篇    下一篇

粗毛纤孔菌形态学鉴定及ITS序列系统发育分析

郭春宣,王峰,董爱荣   

  • 收稿日期:2009-09-10 修回日期:2009-09-15 出版日期:2010-02-05 发布日期:2010-02-05
  • 基金资助:

    黑龙江省重大科技攻关(校企合作)项目

Diagnosis and ITS Phylogenetic Analysis of Inonotus hispidus

  • Received:2009-09-10 Revised:2009-09-15 Online:2010-02-05 Published:2010-02-05

摘要:

2008年于哈尔滨城市林业示范基地采取水曲柳树干着生木材腐朽菌子实体,形态学鉴定为粗毛纤孔菌(Inonotus hispidus)。设计纤孔菌属(Inonotus)真菌ITS特异引物,并进行PCR扩增,扩增产物长500bp。扩增产物测序结果与其它10种纤孔菌属真菌ITS序列构建系统发育聚类树。聚类结果表明粗毛纤孔菌与辐射状纤孔菌亲缘关系近;I. circinatus与I. tomentosus亲缘关系近;薄皮纤孔菌与芮克纤孔菌亲缘关系近;I. dryophilus与I. rheades亲缘关系近。I. circinatus、I. tomentosus、粗毛纤孔菌以及辐射状纤孔菌聚为一族;薄皮纤孔菌、芮克纤孔菌、斜生纤孔菌以及I. glomeratus聚为一族;I. dryophilus、杨生核纤孔以及柽柳核纤孔菌聚为一族。

关键词: 天然彩色棉, 天然彩色棉, 棕色棉, 色素, 棉纤维, 性状

Abstract:

A kind of white rot related fruition bodies on the trunk of Fraxinus mandshurica was diagnosed as Inonotus hispidus by the characters of morphology. Specific polymerase chain reaction (PCR) of the internal transcribed spacer (ITS) region was carried out successfully from DNA extracted from fruiting bodies. The length of the PCR product is 500bp. Phylogenetic analysis of the ITS region for 11 species of Inonotus (10 was from NCBI) was done. The phenogram inferred from the alignment suggested that I. hispidus and I. radiatus were evolutionarily closely related to one another in this study. I. circinatus and I. tomentosus were evolutionarily closely related to one another; I. cuticularis and I. rickii were evolutionarily closely related to one another; I. dryophilus and I. rheades were evolutionarily closely related to one another. I. circinatus, I. tomentosus, I. hispidus and I. radiatus might had the same ancestor; I. cuticularis, I. rickii, I. obliquus and I. glomeratus might had the same ancestor; I. dryophilus, I. rheades and I. tamaricis might had the same ancestor.