欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (8): 226-229.

所属专题： 生物技术； 农业生态

• 植物保护 农药 • 上一篇    下一篇

酶动力学方法在黄曲条跳甲抗性监测中的应用

郑丽祯1,2,傅建炜1,2,李建宇1,林泽燕2,占志雄1   

  • 收稿日期:2009-10-13 修回日期:2009-11-18 出版日期:2010-04-20 发布日期:2010-04-20
  • 基金资助:

    福建省自然科学基金

Application of enzymatic kinetics method in resistance monitoring of striped flea beetle, phyllotreta striolata (Coleoptera: Chrysomelidae)

Zheng Lizhen1,2, Fu Jianwei1,2, Li Jianyu1, Lin Zeyan2, Zhan Zhixiong1   

  • Received:2009-10-13 Revised:2009-11-18 Online:2010-04-20 Published:2010-04-20

PDF (PC)

1225

摘要：

摘要：利用酶标仪动力学方法测定4个菜区黄曲条跳甲的总酯酶、乙酰胆碱酯酶活性分布频率，并与浸叶法测定的药剂敏感性进行比较分析，结果表明利用酶标动力学方法能够快速测定黄曲条跳甲总酯酶、乙酰胆碱酯酶活性在不同活性区段的频率分布，通过分析总酯酶活性大于3.2⊿D(450nm)?min-1?insect-1、乙酰胆碱酯酶活性小于0.016⊿D(405nm)?min-1?insect-1的个体数可以快速监测黄曲条跳甲田间种群的药剂敏感性水平高低。

关键词: 鸡致病性大肠杆菌, 鸡致病性大肠杆菌, O血清型, 药敏试验

Abstract:

Abstract: Frequency distributions of general esterase and acetylcholinesterase in striped flea beetle，phyllotreta striolata，from 4 regions were detected with enzymatic kinetics method in a microplate reader, and were compared with the insecticide susceptibility tested by leaf dipping methods. The results indicated that the former method was a rapid detection of insecticide susceptibility of striped flea beetle by analyzing individual number on the general esterase with an activity of more than 3.2⊿D(450nm)?min-1?insect-1, while acetylcholinesterase less than 0.016⊿D(405nm)?min-1?insect-1.