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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (8): 25-28.

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

不同浓度乙二醇和甘油对犬精液冷冻效果的影响

高雅1,罗桂河2,李卫东3,李复煌4,孟丹娜2,康蕊1,秦秀蕊1,倪和民1   

  • 收稿日期:2009-10-26 修回日期:2009-11-20 出版日期:2010-04-20 发布日期:2010-04-20
  • 基金资助:

    北京市教育委员会科技发展计划重点项目

Influence of Different Concentrations of Ethylene Glycol and Glycerol on Quality of Canine Frozen Sperm

Gao Ya1, Luo Guihe2, Li Weidong3, Li Fuhuang4, Meng Danna2, Kang Rui1, Qin Xiuru   

  • Received:2009-10-26 Revised:2009-11-20 Online:2010-04-20 Published:2010-04-20

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摘要：

目的 研究在常规冷冻稀释液中分别添加不同浓度的乙二醇作为保护剂，并与甘油作为冷冻保护剂进行对比，以优化犬精液冷冻方案，提高解冻后精子质量。方法 在犬的常规精液冷冻稀释液中分别添加不同浓度的乙二醇（2%, 4%, 6%, 8%, 10%），经细管冷冻、解冻后通过分别评价其精子的活率、顶体完整率、畸形率等来筛选其最佳添加浓度。结果 添加浓度为6%的乙二醇时，冷冻后精子活力为0.37，顶体完整率为43%，显著好于其他处理组（P＜0.05）；添加浓度为4%的乙二醇时，冷冻后精子畸形率为15%，略优于其他处理组。结论 6%浓度的乙二醇可代替甘油作为冷冻保护剂，且对犬的冷冻效果有显著的促进作用。

关键词: 蔬菜, 蔬菜, 硝酸盐, 累积, 影响

Abstract:

Objective To optimize the freezing programs for canine semen, the different concentrations of ethylene glycol as protective agent were added in the conventional frozen diluted solution respectively, and compared with the groups added by glycerol as cryoprotectant. Methods The different concentrations of ethylene glycol(2%, 4%, 6%, 8%, 10%) were added in conventional frozen diluted solution of canine semen respectively, the post-thaw semen motility, the percentage of sperms with intact acrosome and the percentage of abnormality were respectively analyzed to estimate the quality of frozen semen. Results The sperm motility in the group with 6% ethylene glycol was 0.37, and its percentage of sperms with intact acrosome was 43%, much higher than those of the other groups(P＜0.05). The percentage of abnormality of 4% ethylene glycol was 15%. Conclusion 6% of ethylene glycol could replace the glycerol as a cryoprotectant, and improve the effect of freezing canine sperm.