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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (6): 146-149.

所属专题： 马铃薯

• 农学 农业基础科学 • 上一篇    下一篇

NaCl胁迫下氮对马铃薯的调控作用

张瑞玖1,2,蒙美莲1,郦海龙1,周长艳1,冯琰1,2   

  • 收稿日期:2009-11-10 修回日期:2009-12-02 出版日期:2010-03-20 发布日期:2010-03-20

Study Nitrogen on Regulation of Potato Under NaCl Stress

  • Received:2009-11-10 Revised:2009-12-02 Online:2010-03-20 Published:2010-03-20

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摘要：

摘 要：以紫花白为试验材料，采用组织培养方法，在离体条件下研究了NaCl胁迫下氮素对马铃薯生理生化特性的影响。主要试验结果如下：NaCl胁迫下马铃薯叶片脯氨酸含量、叶绿素含量、可溶性蛋白含量及根系活力随氮水平的增加表现为先增加后降低的趋势，在4.17（N2）mmol/LNH4NO3水平下达到最大值，其中叶绿素含量、可溶性蛋白含量及根系活力在N2水平下显著的高于对照；NaCl胁迫下马铃薯叶片SOD和POD的活性随氮水平的增加逐渐上升，且在6.25（N3）mmol/LNH4NO3水平下达到最大值。

关键词: 冬枣, 冬枣, 花药, 愈伤组织悬浮系, 原生质体, 分离

Abstract:

Abstract: Zihuabai was used to do an experiment which researched the effects of nitrogen on physiological and biochemical characteristics of potato under NaCl stress in vitro. The results showed: the content of praline、 chlorophyll、protein and root activity performed increasing at first,then decreasing with the increase of nitrogen level，and reached the top in 4.17（N2）mmol/L NH4NO3 level. The content of chlorophyll、protein and root activity were significantly higher than that of the contrast in N2 level; The activity of SOD and POD increased generally under NaCl stress with the increase of nitrogen level，and reached the top in 6.25（N2）mmol/L NH4NO3 level.