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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (10): 212-216.

• 林学 园艺 园林 • 上一篇    下一篇

非洲紫罗兰叶片外植体再生技术体系的建立

程云清,刘剑锋,钟雪,陈智文   

  • 收稿日期:2009-12-21 修回日期:2010-01-10 出版日期:2010-05-20 发布日期:2010-05-20
  • 基金资助:

    国家自然科学基金项目

Tissue Culture and Regeneration System from Leaves of Saintpaulia ionantha in vitro

Cheng Yunqing, Liu Jianfeng, Zhong Xue, Chen Zhiwen   

  • Received:2009-12-21 Revised:2010-01-10 Online:2010-05-20 Published:2010-05-20

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摘要：

以三种叶型的非洲紫罗兰（Saintpaulia ionantha）叶片作为外植体材料，研究了叶片外植体再生技术体系。结果表明：非洲紫罗兰叶片愈伤组织诱导的最佳培养基为MS+6-BA 0.5 mg/L+NAA 0.3 mg/L和MS+6-BA 0.5 mg/L+NAA 0.5mg/L，培养4周左右，诱导率达100%，绝大多数愈伤组织上都有芽的分化。采用培养基MS+6-BA 0.5 mg/L+NAA 0.3 mg/L继代一次后，每个愈伤组织上分化产生的芽可达30余个，3个品种的愈伤组织诱导、成芽能力差异不大。非洲紫罗兰品种A和C适宜的生根培养基为1/2MS+0.2mg?L－1NAA，品种B适宜的生根培养基为1/2MS+0.5mg?L－1IBA。生根苗转入纯蛭石栽培时，用1/4MS大量与微量元素进行叶片追肥，成活率90%以上。移栽至腐殖土栽培成活率95%以上，3个月栽培后栽培苗成花。本研究为非洲紫罗兰试管苗的工厂化生产提供了科学依据。

关键词: 荣宝, 荣宝, 土壤消毒, 作物产量, 效益, 影响

Abstract:

Leaf of three varieties of different leaf-type of African violet（Saintpaulia ionantha）was used as explants to establish a rapid and effective tissue culture regeneration system. The results showed the best culture media of leaf callus induction were MS+6-BA 0.5 mg/L+NAA 0.3 mg/L and MS+6-BA 0.5 mg/L+NAA 0.5mg/L. After 4 weeks cultivation, callus induction rate reached to 100%, and buds occurred on almost every callus. After subculture on the medium MS+6-BA 0.5 mg/L+NAA 0.3 mg/L, about 30 buds could be observed on one callus. There were no obvious differences in callus and bud induction of in different cultivars. The proper culture medium for rooting culture was 1/2MS+0.2mg?L-1 NAA for varieties A and C, but 1/2MS+0.5mg?L－1IBA for B varieties. vermiculite was the optimum medium for test-tube plantlet after transplant, and 1/4 MS(large elements and trace element) should be used as foliar fertilizer, and the survive rate of the root regeneration plant was over 90%. After transferred to humus, above 95% of plantlet could survive. After 3 month of cultivation, the African violet burst into blossom. The results above provided the basic data for industrialized production of test-tube plantlet of African violet.