欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (11): 28-31.

所属专题： 生物技术； 园艺

• 生物技术科学 • 上一篇    下一篇

海南龙血树基因组DNA提取方法比较

董美超1,唐燕琼1,莫饶1,尹俊梅2,张影波1,陈媚1,刘迪发1   

  • 收稿日期:2009-12-28 修回日期:2010-01-08 出版日期:2010-06-05 发布日期:2010-06-05
  • 基金资助:

    农业部热带作物种质资源利用重点开放实验室;海南省自然科学基金

A Comparative Study on Genomic DNA Extraction from Young Leaves of Dracaena cambodiana Pierre ex Gagnepain

Dong Meichao1, Tang Yanqiong1, Mo Rao1,Yin Junmei2, Zhang Yingbo1, Chen Mei1, Li   

  • Received:2009-12-28 Revised:2010-01-08 Online:2010-06-05 Published:2010-06-05

PDF (PC)

1286

摘要：

摘 要：为选择一种简单、快速、有效的海南龙血树总DNA的提取方法，分别采用CTAB法、SDS法、SDS-CTAB法和改良CTAB法提取海南龙血树嫩叶片的总DNA，用琼脂糖凝胶电泳、紫外分光光度计和ISSR对所提取的总DNA进行检测。结果表明，改良CTAB法提取的DNA A260/A280在1.7-1.9之间，纯度高、杂质少、DNA完整性好。以该方法提取的总DNA为模板进行ISSR扩增，谱带清晰，多态性、稳定性、重复性好。该法提取的总DNA适用于PCR扩增和其他分子生物学研究是有效提取海南龙血树基因组的方法。

关键词: 海南, 海南, 橡胶树, 寒害

Abstract:

Abstract：To find a simple,rapid and effective total DNA extraction method for Dracaena cambodiana Pierre ex Gagnepain,four methods, CTAB、SDS、SDS-CTAB、Improved CTAB method,were compared.The total DNA was detected by agarose electrophoresis,Spectrophotometer and ISSR amplification.The result showed that the A260/A280 of total DNA extracted by the Improved CTAB method ranged from 1.7 to 1.9 with high-purity,low levels of impurity and good integrity.ISSR fragments amplified with DNA template extracted by Improved CTAB method showed clear bands,and good polymorphic,stability and repetition.The Improved CTAB method was effective for total DNA extraction Dracaena cambodiana Pierre ex Gagnepain,which was suitable for PCR amplification and other molecular biology researches.