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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (13): 23-29.

所属专题： 生物技术； 园艺

• 生物技术科学 • 上一篇    下一篇

乙醛脱氢酶基因（ALDH）转化番茄的研究

张海玲 张月学 尚晨 李佶恺 徐香玲   

  • 收稿日期:2010-01-04 修回日期:2010-01-22 出版日期:2010-07-05 发布日期:2010-07-05

Studies on Transformation of Aldehyde dehydrogenase (ALDH) into Tomato

  • Received:2010-01-04 Revised:2010-01-22 Online:2010-07-05 Published:2010-07-05

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摘要：

乙醛脱氢酶基因（ALDH）为目的基因，构建了pBI-ALDH 植物表达载体，番茄品系03HN31子叶为外植体，经发根农杆菌（Agrobacterium rhizogens）介导采用共培养法转化番茄。通过分子生物学技术（PCR、Southern杂交、RT-PCR）和抗逆相关生理指标测定（相对电导率及丙二醛含量）相结合的方法对转化番茄植株进行检测，研究结果表明：乙醛脱氢酶基因（ ALDH ）导入并整合到番茄的基因组中，在转录水平上可以稳定表达；在干旱、高盐和低温胁迫条件下，转基因番茄植株和对照株的相对电导率和丙二醛含量有差异，证明转化番茄植株的质膜受损程度有所降低，抗氧化胁迫性能有所提高，转化率为10.78%。

关键词: 技术创新, 技术创新, 农业, 烟叶, 可持续发展

Abstract:

The aldehyde dehydrogenase (ALDH) was studied as target gene in this study, and the plant expression vector pBI-ALDH has been constructed.Tomato 03HN31 Cotyledon was transformed by A.rhizogenes R1000. We detected the transformed plants by multiple molecular biology methods (PCR, outhern blot and RT-PCR) and resistance-correlated physiological indices (relative electric conductivity and malondialdehyde content). Results showed that the ALDH gene had been integrated into the genome of tomato and expressed at transcriptional level. Moreover, the relative electronic conductivity and malondialdehyde (MDA) content showed different between transformed tomato plants and controls under stresses of drought, high-salt and hypothermia etc, which indicated that the damage of transformed plants plasma membrane was decreased the antioxidant capacity was increased under stress. The transformation ratio is 10.78%.