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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (11): 92-98.

所属专题： 小麦

• 农学 农业基础科学 • 上一篇    下一篇

金荞麦辐射诱变变异株系光合参数变化及其与环境因子关系

兰金旭,陈彩霞,杨念婉,谢赛萍,李艾莲   

  • 收稿日期:2010-01-05 修回日期:2010-01-26 出版日期:2010-06-05 发布日期:2010-06-05
  • 基金资助:

    金荞麦辐射诱变类黄酮次生代谢相关基因功能分析

Changes of Photosynthetic Parameters in Leave of Fagopyrum dibotrys Mutants and Its Relation to Environmental Factors

Lan Jinxu, Chen Caixia, Yang Nianwan, Xie Saiping, Li Ailian   

  • Received:2010-01-05 Revised:2010-01-26 Online:2010-06-05 Published:2010-06-05

PDF (PC)

1016

摘要：

为了探讨金荞麦辐照诱变株系在快速生长期的光响应和光合日变化规律，培育新种质，以Co60γ辐照绿茎金荞麦根茎（对照）及得到的红茎、红叶诱变株系植株为试验材料，利用Li-6400便携式光合测定仪连续2年进行了光合参数测定。结果表明：红叶和红茎株系在弱光下具有较好的光利用能力，而绿茎株系在强光下具有较强的光合作用能力；三种金荞麦的净光合速率日变化均呈双峰曲线，由于气孔限制的影响，具有“光合午休”现象；日光合进程中，绿茎的光合速率高于红茎和红叶；光照和温度是影响金荞麦光合作用的主要影响因素，红茎的光合作用需要高CO2浓度，而高CO2浓度却抑制了绿茎和红叶的光合作用，低湿也是影响红叶光合作用的重要因子。研究结果说明金荞麦变异株系的光合能力明显弱于绿茎对照植株。

关键词: 地黄, 地黄, RNA提取, 抑制消减杂交, RACE

Abstract:

The light response curve and diurnal variation of net photosynthetic rate of different Fagopyrum dibotrys mutants bred by Co60γ irradiation was studied during rapid growth period for developing new germplasms by the Li-6400 portable photosynthetic system. The results showed that the red leaves and red stems had higher photosynthetic capacity under low light, but the green stem had higher photosynthetic capacity under high light; the diurnal net photosynthetic rate of three Fagopyrum dibotrys sunny day exhibited double-peak pattern, which was caused principally by stomatal limitation and midday depression were appeared; the diurnal net photosynthetic rate of green stem are higher than red stem and red leaves; light and higher temperature was main factor that affected the Fagopyrum dibotrys photosynthetic rate, red stem needs higher carbon dioxide concentration, but that suppress green stem and red leaves, and lower relative humidity was another factors affected red leaves photosynthetic rate. The researches indicated Fagopyrum cymosum mutants had lower photosynthesis capacity than original plants.