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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (12): 40-44.

所属专题： 生物技术； 食用菌； 食用菌

• 食品 营养 检测 安全 • 上一篇    下一篇

一氧化氮对双孢蘑菇采后生理及贮藏品质的影响

张少颖   

  • 收稿日期:2010-01-28 修回日期:2010-02-25 出版日期:2010-06-20 发布日期:2010-06-20
  • 基金资助:

    山西师范大学博士科研启动基金

Effect of nitric oxide on postharvest physiology and quality of Agaricus bisporus

Zhang Shaoying   

  • Received:2010-01-28 Revised:2010-02-25 Online:2010-06-20 Published:2010-06-20

PDF (PC)

1131

摘要：

以硝普钠（SNP）为一氧化氮供体，研究了不同浓度NO在常温（25±1℃）和低温（4±1℃）条件下对双孢蘑菇‘F56’贮藏过程中相关品质（失重率、开伞率、可溶性固形物、维生素C（Vc）、游离氨基酸含量）以及过氧化氢酶（CAT）、过氧化物酶（POD）和多酚氧化酶（PPO）活性的影响。结果表明，0.09 mmol/L SNP释放的NO显著抑制了双孢蘑菇采后失重率和游离氨基酸含量的上升，减缓了可溶性固形物含量的增加、延缓Vc的降解，抑制了开伞率的增大；使CAT、POD的活性显著增强，同时降低PPO活性。因此，0.09mmol/L SNP处理显著提高了常温和低温下双胞蘑菇‘F56’ 贮藏期间的CAT、POD活性，抑制了PPO活性的升高，延缓了菇体品质的下降。

关键词: RNA提取, RNA提取, 甘薯

Abstract:

Using sodium nitroprusside (SNP) as a nitric oxide (NO) donor, the effects of different concentrations of nitric oxide treatment on physiological index of post-harvest Agaricus bisporus ‘F56’ ,at room temperature (25 ± 1 ℃) and low temperature (4 ± 1 ℃), including weight loss, line rate,total soluble solid shape structures, vitamin C(Vc), free amino acid content ,and activities of relevant enzymes from Agaricus visporus, including catalase (CAT), peroxidase (POD) and polyphenol oxidase (PPO) , were investigated. The result showed NO resulting from 0.09mmol/L SNP aqueous solution obviously inhibited weight loss, line rate, the increase of soluble solid shape structures, free amino acid content and delayed the decrease of vitamin C. The activities of CAT and POD obviously increased, and the activity of PPO decreased during storage for Agaricus visporus treated with 0.09 mmol/LSNP.Consequently, 0.09 mmol/L SNP treatment can significantly increase the activities of CAT and POD, inhibit the activity of PPO of Agaricus bisporus ‘F56’at room temperature and low temperature,and slow the decline in sporophore quality.