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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (14): 172-176.

所属专题： 生物技术； 水稻

• 农学 农业基础科学 • 上一篇    下一篇

粳稻和籼稻愈伤诱导和分化期间生理代谢差异初探

张湘玲 陶跃之 宋红苗   

  • 收稿日期:2010-02-10 修回日期:2010-03-24 出版日期:2010-07-20 发布日期:2010-07-20
  • 基金资助:
    水稻产量相关基因的功能基因组学研究

Preliminary analysis of physiological metabolism of callus induction and differentiation between Japonica and Indica rice (Oryza sativa L.)

  • Received:2010-02-10 Revised:2010-03-24 Online:2010-07-20 Published:2010-07-20

PDF (PC)

1067

摘要： 通过比较粳稻品种日本晴和籼稻品种9311成熟胚愈伤诱导和分化阶段，愈伤组织的基础生理代谢差异，探讨籼稻9311再生率低于粳稻日本晴的可能原因。研究表明，在愈伤诱导和分化的所有不同时期，在所有时期日本晴愈伤中的总氮和氨态氮含量均高于9311，在分化25天以前，硝态氮含量也高于9311。此外，在细胞代谢水平上，通过对可溶性蛋白含量的差异分析表明，在愈伤诱导一个月至分化15天时，日本晴的可溶性蛋白含量高于9311。

关键词: betA, betA, BADH, 酶切, PCR

Abstract: Possible causes for poorer regeneration of indica variety 9311 than japonica variety Nipponbare were analized by comparing the differences of physiological metabolism in callus, which obtained at different stages of callus induction and differentiation. The results demonstrate that, in different culture days of callus induction and differentiation, total nitrogen and ammoniacal nitrogen in Nipponbare callus is higher than that of 9311. Nitrate nitrogen in Nipponbare is also higher than that in 9311 before 25days of callus differentiation. Moreover, at the level of cells metabolism, soluble protein in Nipponbare is higher than that in 9311 from callus induction to 15 days of differentiation.