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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (19): 339-343.

• 资源 环境 生态 土壤 气象 • 上一篇    下一篇

氯嘧磺隆降解菌L-6的分离鉴定及其降解特性

刘艳 范丽薇 王晓萍   

  • 收稿日期:2010-04-07 修回日期:2010-05-10 出版日期:2010-10-05 发布日期:2010-10-05

Isolation Identification and Degradation Characteristics of Chlorimuron-ethyl Degradation Bacteria L-6

  • Received:2010-04-07 Revised:2010-05-10 Online:2010-10-05 Published:2010-10-05

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摘要：

利用富集培养技术从长期施用氯嘧磺隆的土壤中分离得到1株能够降解氯嘧磺隆的细菌L-6。通过生理生化特性和16SrDNA序列分析，初步鉴定L-6为恶臭假单胞菌(Pseudomonas putida)。并分析了氯嘧磺隆的初始浓度、接种量、温度和PH值对L-6菌株降解氯嘧磺隆效果的影响，确定了最佳降解条件。结果显示，该菌在氯嘧磺隆浓度为200 mg/L、接种量为10％、PH8.0、温度30℃条件下，接种4 d后对氯嘧磺隆的降解效率达到80％以上。这表明该菌株具有降解氯嘧磺隆的潜在应用价值。

关键词: PKS, PKS, NRPS, 数据库, 生物信息学

Abstract:

In order to provide the theoretic basis in bioremediation of chlorimuron-ethyl-contaminated soil, a chlorimuron-ethyl degradable strain was obtained from chlorimuron-ethyl-contaminated soil. L-6, a bacterium which was able to degrade chlorimuron-ethyl, was isolated from the soil of long term applied with chlorimuron-ethyl by enrichment culture. Based on morphology observation, physiological and biochemical characteristics, 16S rDNA sequence analysis, the strain L-6 was identified preliminarily asPseudomonas putida. The effect of initial concentration of chlorimuron-ethyl, inoculation amount, temperature and pH on degradation efficiencies was studied. The optimal degrading conditions were: initial concentration of chlorimuron-ethyl 200 mg/L, inoculation amount of 10%, pH 8.0, respectively, under the optimal conditions, the degrading efficiency could reach more than 80% after 4 days at 30℃. These results indicated that the strain L-6 had certain potential of degrading chlorimuron-ethyl.