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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (22): 381-384.

• 农学 农业基础科学 • 上一篇    下一篇

利用巢式PCR对海南槟榔（Areca catechu L.）黄化病的初步检测

周亚奎 甘炳春 张 争 隋 春 魏建和 杨 云 杨新全   

  • 收稿日期:2010-04-13 修回日期:2010-06-08 出版日期:2010-11-20 发布日期:2010-11-20
  • 基金资助:

    “十一五”国家科技支撑计划课题“重点道地南药良种选育及规范化生产关键技术研究”

Detection of the phytoplasmas associated with yellow leaf disease of Areca catechu L. in Hainan province of China by nested PCR

  • Received:2010-04-13 Revised:2010-06-08 Online:2010-11-20 Published:2010-11-20

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摘要：

槟榔黄化病是世界各地槟榔生产上普遍发生的毁灭性病害。至今没有根治的有效方法，根本原因是其病原还没有明确鉴定。有报道表明槟榔黄化病的病原可能是植原体。本文利用巢式PCR方法，对海南3个市县采集的28个黄化病植株的不同器官进行了植原体检测，结果表明在2市县9个槟榔黄化病病株叶片中检测到了植原体，而在根和茎中没有发现植原体。同源性聚类发现其属于翠菊黄花组B亚组。

关键词: 淹水, 淹水, NO3-, 叶绿素荧光

Abstract:

Yellow leaf disease (YLD) of the areca nut palm is a severe disease that occurs mainly in Southeast Asia. Although YLD has similar symptoms in different regions, it has been shown to be induced by different pathogens. In this work, 28 samples of diseased palms were collected from three different counties of Hainan Province, China. Their 16S rDNA was studied by the nested PCR to detect the phytoplasma associated with YLD. The results showed that amplification was observed only in leaf samples, but not in stem or root samples, and phytoplasmas were detected only in 9 samples collected from 2 counties of Hainan pronvices.