欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (22): 213-217.

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

不同磷钙元素水平番茄营养液中细菌多样性

雷娟利 寿伟松 董文其 张成浩 徐志豪 周艳虹 喻景权   

  • 收稿日期:2010-04-19 修回日期:2010-07-19 出版日期:2010-11-20 发布日期:2010-11-20
  • 基金资助:

    绿色环控设施农业关键技术研究与产业示范

Bacterial diversity in tomato hydroponic culture solution with different element Ca and P level

  • Received:2010-04-19 Revised:2010-07-19 Online:2010-11-20 Published:2010-11-20

PDF (PC)

1185

摘要：

采用从营养液中直接提取微生物总DNA，并用细菌16S rDNA 特异性引物进行PCR 扩增、变性梯度凝胶电泳（DGGE）、克隆和测序等一系列分子生物学手段，对不同磷钙元素水平番茄营养液中细菌多样性进行了研究。结果表明，各种元素水平的番茄营养液，均有相似的条带，只是亮度上有些差异。与对照相比，各处理元素水平营养液中的细菌DGGE条带均有变化。对照DGGE的条带分布相对均匀，而其它样品的DGGE条带分布多不均匀。测序结果表明，番茄营养液中的细菌类群主要包括：Proteobacteria（Alpha，Beta，Gamma和Unclassified），Bacteroidetes和Gram Positive Bacteria。

关键词: 秸秆功能, 秸秆功能, 秸秆还田, 生态效应, 秸秆腐解剂

Abstract:

Using DNA directly extracted, bacterial 16S rDNA PCR amplification, DGGE, cloning and sequencing methods, to study the bacterial diversity in tomato hydroponic culture with different element Ca and P level, indicated interesting results. The results showed that each hydroponic culture of element level had the similar DGGE bands patterns. Compared with the control, every DGGE band patterns of the hydroponic culture changed differently. The bands distribution in the control was more evenly than that of the treatments. Sequencing results showed that the main bacterial divisons in tomato hydroponic culture were Proteobacteria（Alpha，Beta，Gamma and Unclassified），Bacteroidetes and Gram Positive Bacteria.