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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (24): 47-51.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

银杏愈伤组织诱导与生长的优化试验

胡燕梅,夏景波,韩晓红,周全   

  • 收稿日期:2010-04-22 修回日期:2010-06-05 出版日期:2010-12-20 发布日期:2010-12-20

Optimization of the Ginkgo Callus Induction and Growth

  • Received:2010-04-22 Revised:2010-06-05 Online:2010-12-20 Published:2010-12-20

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摘要：

采用正交试验研究了四种外植体、基本培养基、萘乙酸（NAA）、6-苄基嘌呤（6-BA）4因素4水平及银杏幼叶汁和胚乳汁对银杏愈伤组织诱导及生长的影响。结果表明：银杏愈伤组织诱导和生长的最佳组合均是以子叶为最佳外植体，而培养基最佳组合分别是MS+ NAA 3.0 mg／L + BA 2.0 mg／L和SH+ NAA 3.0 mg／L + BA 1.5 mg／L；四种因子影响次序是外植体 ＞基本培养基＞NAA ＞ 6-BA；银杏胚乳汁促进愈伤组织生长，而银杏叶汁却抑制愈伤组织生长。

关键词: 台湾, 台湾, 食品安全, 监管, 质量认证

Abstract:

Ginkgo biloba L. callus induction and growth were studied by orthogonal test of different combinatibns of four explants ，minimal medium ，NAA and 6 –BA ，and two other factors of ginkgo leaf juice and endosperm juice. The results showed ：the optimum　combinations were MS+ NAA 3.0 mg／L + BA 2.0 mg／L for callus induction and SH+ NAA 3.0 mg／L + BA 1.5 mg／L for callus growth with cotyledon as explant. Effect sequence of four effects was explants ＞minimal medium＞NAA ＞ 6-BA for callus induction and growth； Ginkgo endosperm juice improved the growth，but leaf juice inhibited the growth of callus.