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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (24): 56-60.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

北极狐增食欲素基因结构特征及多态性与生产性能相关性分析

张敏 白秀娟   

  • 收稿日期:2010-04-26 修回日期:2010-05-30 出版日期:2010-12-20 发布日期:2010-12-20

Arctic Fox orexin gene structure features and polymorphism analysis and production of performance-related

  • Received:2010-04-26 Revised:2010-05-30 Online:2010-12-20 Published:2010-12-20

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摘要：

摘要： 本研究针对大兴安岭地区北极狐增食欲素基因结构特征进行分析并且通过PCR-SSCP方法寻找影响北极狐生产性能的增食欲素基因外显子突变位点。数据利用SignalP 3.0 Server 、 ClustalX1.83、Mega4.0、Promoter predictions、TMHM2.0、 ProtComp-v6.0等生物学软件和SPSS13.0软件完成分析，结果北极狐与狗的遗传距离是最近，增食欲素它可能是一个分泌蛋白，具有一个信号肽的序列的可能性很大，增食欲素基因外显子中存在多态，但是个基因型之间没有显著型的差异。

关键词: 晋谷, 晋谷, 蛋白质, 氨基酸, 营养评价

Abstract:

Abstract： In this study, arctic foxes Daxinganling region orexin gene structure features were analyzed through PCR-SSCP method to find the impact performance of arctic foxes orexin gene exon mutations. Data using SignalP 3.0 Server, ClustalX1.83, Mega4.0, Promoter predictions, TMHM2.0, ProtComp-v6.0 software and SPSS13.0 software such as biology to complete the analysis, the results of Arctic foxes and dogs genetic distance is the latest, by Orexin it may be a secreted protein with a signal peptide sequence is considerable, and orexin gene exon exists in many states, however, between genotypes no significant differences in type