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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (24): 221-225.

• 植物保护 农药 • 上一篇    下一篇

威灵仙黑斑病的病原鉴定及防治药剂的离体活性筛选

蒋妮 黄永才 唐美琼 林杨 刘威 覃柳燕   

  • 收稿日期:2010-05-25 修回日期:2010-07-06 出版日期:2010-12-20 发布日期:2010-12-20
  • 基金资助:
    广西道地中药材威灵仙野生变家种技术研究

Identification of Clematis chinensis Black Spot and Screening of Effective Fungicides

  • Received:2010-05-25 Revised:2010-07-06 Online:2010-12-20 Published:2010-12-20

摘要： 为明确威灵仙黑斑病的致病菌并筛选出有效的防治药剂，采用形态特征及菌株的ITS序列分析方法进行病原菌鉴定，通过平皿菌丝生长抑制法测定杀菌活性，结果表明威灵仙黑斑病病原菌为Alternaria alternata (Fr.)Keissler；25%溴菌腈可湿性粉剂、72%霜疫力克可湿性粉剂、30%爱苗乳油、80%绿亨2号可湿性粉剂和10%世高水分散粒剂对菌丝抑制率均达90％以上。此研究结果可为该病害的有效控制提供前期研究基础。

关键词: 苹果, 苹果, 原生质体分离, 原生质体纯化, 原生质体培养

Abstract: Clematis chinensis black spot severely occurred in Jingxi, Guangxi. An pathogen was isolated from diseased lesions on the leaves of Clematis chinensis ,and its pathogenicity to Clematis chinensis confirmed by inoculation tests. The pathogen was identified as Alternaria alternata (Fr.) Keissler based on its morphological characteristics and ITS sequence. Screening tests of the fungicides indicated that 25% bromothalonil WP, 72% Mancozeb+cymoxanil WP, 30% Armure EC, 80% thiram+Zinc bis dimethyldithiocarbamate WP, 10% difenoconazole WDG all showed good inhibition against mycelial growth, with inhibition rate greater than 90%. This is the first record of Clematis chinensis black spot caused by A. ahernata in Guangxi.