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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (1): 214-218.

所属专题： 油料作物

• 生物技术科学 • 上一篇    下一篇

花生总RNA提取方法比较研究

陈 高 单 雷 周立侠 唐桂英 毕玉平   

  • 收稿日期:2010-06-22 修回日期:2010-08-13 出版日期:2011-01-07 发布日期:2011-01-07

The Comparison of Different Methods for Isolating Total RNA from Peanuts

  • Received:2010-06-22 Revised:2010-08-13 Online:2011-01-07 Published:2011-01-07

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摘要：

通过对2种RNA提取方法（改良CTAB法和Trizol法）和2种RNA提取试剂盒（AXYGEN总RNA提取试剂盒和TIANDZ总RNA提取试剂盒）进行比较研究，以期获得质量较好的花生不同器官的总RNA，为后续分子生物学实验奠定基础。实验结果表明，与TRIZOL法及2种RNA提取试剂盒相比，改良CTAB法提取的花生总RNA完整性好，得率高，稳定性好，并且无DNA污染，可以进一步满足半定量RT-PCR等实验需要。

关键词: 鸡, 鸡, MOB基因, 克隆, 生物信息学, SAM结构域

Abstract:

In order to obtain better quality total RNA in different parts of peanuts, two RNA extraction methods (CTAB and Trizol) and two RNA extraction kits (AXYGEN and TIANDZ) were compared. The results showed that the improved CTAB method was better than Trizol method and two total RNA extraction kits. The total RNA isolated with the improved CTAB method had high integrity, high yield, good stability and no DNA pollution, and then could be used for semi-quantitative RT-PCR and so on.

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