欢迎访问《中国农学通报》,

中国农学通报 ›› 2010, Vol. 26 ›› Issue (20): 406-412.

所属专题: 生物技术 马铃薯

• 生物质能源 • 上一篇    下一篇

木薯生料发酵转化乙醇的工艺参数优化研究

蔡柳 谭显胜 袁哲明 苏小军 张婷婷 李清明 熊兴耀   

  • 收稿日期:2010-06-28 修回日期:2010-07-19 出版日期:2010-10-20 发布日期:2010-10-20
  • 基金资助:

    国家科技支撑项目;教育部新世纪优秀人才计划

Optimization of Technological Parameters for Ethanol Production by Fermentation of Raw Cassava

  • Received:2010-06-28 Revised:2010-07-19 Online:2010-10-20 Published:2010-10-20

摘要:

以木薯(Manihot esculenta)为试材,分别用正交设计法和均匀设计法考察木薯生料发酵转化乙醇工艺参数,分析讨论了这两种设计的特点,得出在多水平多因素的条件下,均匀设计具有实施试验少、效果好等优势,并得到以乙醇产量为指标的生料发酵转化乙醇最佳工艺参数:料水比1:1.8,初始pH3.5,温度32℃,酵母添加量3.5×107cell/mL,(NH4)2SO4 0.5g,转速140r/min,糖化酶添加量200 u/g,淀粉酶添加量12 u/g,纤维素酶添加量25 u/g, 发酵周期120 h,此时乙醇得率达到15.7%(正交设计最优结果为9.2%)。

关键词: 组织管理, 组织管理, 经验, 问题, 对策

Abstract:

Taking cassava as test piece,employ the uniform design and orthogonal design studying in the technological parameters optimizated for raw cassava ethanol production,and discussed the two design’s methods characteristics,The results uniform design has more advantages than orthogonal design under the conditions of multi-levels and multi-factors;The optimal technological parameters for the ethanol yield were obtained: Ratio of solid to liquid 1:1.8, initial pH value 3.5,fermentation temperature 32℃,yeast inoculation amount 3.5×107cell/mL,(NH4)2SO4 0.5g,rotational speed 140rpm,dosage of gluloamylase , a-amylase and cellulase were 200 u/g,12 u/g and 25 u/g,fermentation cycle 120h,under the optimal conditions,ethanol concentration could reach 15.7%(9.2% under orthogonal design).