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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (18): 63-66.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

PEG对杂花苜蓿体胚发生的影响及体胚的细胞学观察

杨国锋   

  • 收稿日期:2010-07-05 修回日期:2010-08-03 出版日期:2011-09-20 发布日期:2011-09-20
  • 基金资助:
    小反刍兽疫植物疫苗初步研究

Effects of PEG on Somatic Embryogenesis of Variegated Alfalfa and Cytological Observation of Somatic Embryo

  • Received:2010-07-05 Revised:2010-08-03 Online:2011-09-20 Published:2011-09-20

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摘要： 以甘农1号杂花苜蓿下胚轴为材料诱导愈伤组织，愈伤组织在添加不同PEG6000浓度的UM+0.1mg/L NAA+0.5mg/L KT培养基上发生体胚，研究PEG6000对体胚发生的影响，并观察了体胚的细胞学结构。结果表明，适量的PEG6000对体胚的发生有促进作用，其中添加6% PEG6000体胚的发生率最高；石蜡切片观察得出体胚的发育经过球形胚、心形胚、鱼雷胚及子叶胚4个阶段形成一个完整的体胚。

关键词: 油棕产业, 油棕产业, 调查, 分析, 建议

Abstract: The callus induced from the hypocotyl in Medicago varia cv. Gannong No.1, was cultured in the UM medium supplied with the PEG6000 of various concentrations, NAA (0.1mg/L) and KT (0.5mg/L), to induce the somatic embryos. Then the study on effect of PEG6000 on the somatic embryogenesis and observation on cytological structure of embryo were carried out in this paper. The results show that, the regeneration medium with proper concentration of PEG6000 are advantageous to somatic embryogenesis, the highest frequency of somatic embryogenesis appeared in regeneration medium with 6%PEG6000; via observed the paraffin sections, we found forming a whole somatic embryo will go through four developing phases, heart-shape embryo, torpedo embryo and cotyledon-shape embryo.