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中国农学通报 ›› 2010, Vol. 26 ›› Issue (18): 67-70.

• 生物技术科学 • 上一篇    下一篇

不同割手密杂交后的父性遗传分析

刘洪博 桃联安 懂立华 安汝东 吴才文 经艳芬   

  • 收稿日期:2010-04-26 修回日期:2010-05-10 出版日期:2011-09-20 发布日期:2011-09-20
  • 基金资助:
    云南省自然科学基金项目

Analysis the male's heredity of hybrids F1 from different S.spontaneum

  • Received:2010-04-26 Revised:2010-05-10 Online:2011-09-20 Published:2011-09-20

摘要: 本研究主要选用云割82-114、云南1号、云割83-184、云割82-25、云割82-160、瑞割3号等优良甘蔗细茎野生种作为父本,利用百眉蔗、粤糖93-159等作为母本,通过有性杂交,并利用SSR鉴定杂交后代的遗传关系,从140个后代材料中鉴定出109个真实杂种,27个是假阳性材料,4个自交材料。并对12个组合真实性达到6个以上的子代进行了父性遗传的评估,结果父本共检测出24个位点,各子代群体的平均父性多态性比率在33.33 %-86.67%之间,平均基因杂合度较低,而父性遗传率相对较高。

关键词: 小型底栖动物, 小型底栖动物, 分布, 环境因子, 南黄海

Abstract: In order to know the genetic relationship of progenies from 15 cross combinations got by sexual hybridization (CYG82-114、 CYG No.1、 CYG83-184、CYG82-25、 CYG82-160 and CRG No.3 were selected as male parent ,Baimei sugarcane、CYT 93-159 et al as female parent), 140 progenies were identified by SSR markers, the result showed 109 clones were true hybrids progenies and 27 clones are false progenies, 4 clones belong to self-fertility progenies. According to fathership genetic evaluations, the progenies of 12 cross combinations ( more than 6 clones at least) liked male parent, their band rate account for the band from male parent range from 33.33% -86.67%,and represented low gene heterozygosity, at the same time, they possess relative high genetic rates of male parent.