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中国农学通报 ›› 2010, Vol. 26 ›› Issue (18): 71-74.

所属专题: 生物技术 油料作物 小麦

• 生物技术科学 • 上一篇    下一篇

离子束介导大豆DNA导入小麦的蛋白质含量稳定分析

苌收伟   

  • 收稿日期:2010-01-14 修回日期:2010-02-11 出版日期:2011-09-20 发布日期:2011-09-20

Analysis of the protein content genetic stability of transgenic wheat

  • Received:2010-01-14 Revised:2010-02-11 Online:2011-09-20 Published:2011-09-20

摘要: 摘要:为了提高小麦蛋白质含量,采用离子束介导法将大豆(Glycine max)豫豆23号的基因组DNA导入小麦(Triticum aestivum L.)品种中育5号和淮阴9628。获得T1代高蛋白含量(>15%)单株114株,蛋白质含量最高达21.44%,显著提高了小麦蛋白质含量。从T1到T3代连续单株选择,获得了16个遗传上基本稳定的T3代株系。结果表明,离子束介导的大豆基因组导入是小麦高蛋白含量改良的可行途径。

关键词: 高粱, 高粱, 体细胞无性系变异, 诱变, 品种改良

Abstract: Abstract:The method of low energy ion beam-mediated transformation was adopted to transfer the soybean DNA into two wheat varieties ‘Zhongyu 5’and ‘Huaiyin 9628’.The results suggested that 114 high-protein individuals (protein content >15%) were obtained from T1, and the highest protein content reached 21.44%. The protein content of wheat individuals was greatly increased. The high-protein character could be mostly inherited from T1 to T3, and the character of some lines in T3 was stable basically. It was showed that the method of low energy ion beam-mediated transformation obviously enhanced the transgene and mutagenic effect, and the variant character could be inherited stably. So this method may become to a most efficient way to realize the breakthough of wheat breeding.