关键词: 高通量测序, 高粱, 盐碱地, 土壤细菌组成、丰度, 多样性分析

Abstract:

To study the changes in soil microbial community structure in saline-alkali soils, and the number of bacteria can be used as one of the criteria for screening sorghum salt-tolerant materials. This study will provide a theoretical basis for the selection of sorghum salt-tolerant materials and the study of sorghum salt tolerance. In the experiment, the Illumina Miseq high-throughput sequencing method was used to analyze the structure of the rhizosphere flora of sorghum in saline-alkali land. Ten different sorghum parent materials were selected to collect rhizospheric soil samples of sorghum in saline-alkali land. The increase in the number of microorganisms and their growth rate can effectively promote the activity of soil enzymes, thereby increasing the growth rate of the material; the Chao1, ACE and Shannon indexes of samples 342 and 473 are the highest, and the Simpson index is the smallest. The species diversity is the highest; on the contrary, the Chao1, ACE and Shannon indexes of the salt-sensitive materials 68 and 622 are the lowest, the Simpson index is the largest, and the diversity is the lowest. The research results show that the rhizosphere soil bacteria of salt-tolerant materials have high species diversity, and this index can be used as an evaluation index for screening salt-tolerant materials.

Key words: high-throughput sequencing, sorghum, saline soil, soil bacterial composition and abundance, diversity analysis