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中国农学通报 ›› 2020, Vol. 36 ›› Issue (36): 22-25.doi: 10.11924/j.issn.1000-6850.casb20200200084

• 农学·农业基础科学 • 上一篇    下一篇

EMS诱变甜高粱突变体筛选与鉴定

张伟(), 赵威军, 白文斌, 张阳, 王官, 邵荣峰, 薛丁丁   

  1. 山西省农业科学院高粱研究所,山西晋中 030600
  • 收稿日期:2020-02-05 修回日期:2020-03-13 出版日期:2020-12-25 发布日期:2020-12-23
  • 作者简介:张伟,男,1989年生,山西柳林人,助理研究员,硕士,主要从事甜高粱遗传育种研究,通信地址:030600 山西省晋中市榆次区蕴华西街山西省农业科学院高粱研究所,E-mail: sxzwei1989@163.com
  • 基金资助:
    高粱遗传与种质创新山西省重点实验室青年基金项目“秋水仙素诱导甜高粱染色体加倍效应研究”(2018Q-4)

Screening and Identification of EMS Mutants from Sweet Sorghum

Zhang Wei(), Zhao Weijun, Bai Wenbin, Zhang Yang, Wang Guan, Shao Rongfeng, Xue Dingding   

  1. Sorghum Institute, Shanxi Academy of Agricultural Sciences, Jinzhong Shanxi 030600
  • Received:2020-02-05 Revised:2020-03-13 Online:2020-12-25 Published:2020-12-23

摘要:

为了创制优良甜高粱种质资源,改良高配合力父本性状,为甜高粱遗传育种奠定基础,本试验利用24 h+0.25%甲基磺酸乙酯(EMS)水溶液诱变甜高粱甜C-1,从M2代中筛选鉴定优良突变体用于下一步遗传育种。研究表明叶色突变主要发生在M1代,且大部分不能遗传,M1代发现的突变,M2代中依然能够大量发现同类突变。在M2代田间鉴定中发现的突变体类型主要有叶色白化和黄化突变、穗型突变为纺锤型、颖壳包被度变异为1/2和3/4包被、颖壳颜色突变为黑褐色、籽粒增大、籽粒突变为白色、芒性消失、全生育期缩短。利用EMS诱变可以改良现有优良父本性状,加速杂交种的选育,且发现大量的突变体对甜高粱基因功能的挖掘和遗传育种有积极的意义。

关键词: 甜高粱, EMS诱变, 叶片突变, 穗部突变, 生育期

Abstract:

To create good germplasm resources of sweet sorghum, improve the male parent characters with high combining ability, and lay a foundation for genetic breeding, ethyl methyl sulfonate (EMS) was used to mutate sweet sorghum restorer line Tian C-1, and the excellent mutants were screened and identified from M2 generation for further genetic breeding. The results show that leaf color mutations mainly occur in M1 generation, and most of them cannot be inherited. The mutations found in M1 generation could be found in M2 generation after single spike harvest. The mutant types found in the field identification of M2 generation mainly include albino mutation and yellowing mutation of leaf color, spindle mutation of spike type, 1/2 and 3/4 glume coat variation, glume color mutation into dark and brown, grain enlargement, grain color mutation into white, awn disappearance, and shortened whole growth period. EMS mutagenesis could improve existing excellent male parent properties, accelerate the breeding of hybrid, and the large number of mutants found in field could have positive significance in genome mining and genetic breeding of sweet sorghum.

Key words: sweet sorghum, EMS mutation, leaf mutation, spike mutation, growth period

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