欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (8): 123-127.

所属专题： 生物技术； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

玫瑰RdGASA4-like基因启动子的分离及其在甘蓝中的瞬时表达分析

张强 张晓伟 孟月娥 李艳敏 王慧娟 王利民   

  • 收稿日期:2010-07-23 修回日期:2010-08-27 出版日期:2011-04-20 发布日期:2011-04-20
  • 基金资助:

    河南省基础与前沿技术研究计划项目

Isolation and Transient Expression Analysis in Cabbage of the Promoter of RdGASA4-like from Rosa damascene

  • Received:2010-07-23 Revised:2010-08-27 Online:2011-04-20 Published:2011-04-20

PDF (PC)

1192

摘要：

在前期的研究中，一个玫瑰RdGASA4-like基因的cDNA和DNA序列全长利用同源克隆结合RACE的方法首次得到克隆。为深入了解该基因在赤霉素调控植物发育过程中的作用，通过TAIL-PCR的方法首次克隆该基因起始密码子ATG上游685bp的5’-UTR和部分启动子序列，在启动子序列分析的基础上，构建其与GUS基因融合表达载体，命名为pBI-GP685。通过农杆菌介导的方法对甘蓝外植体子叶-子叶柄进行启动子瞬时表达研究，结果表明分离得到的启动子具有启动报告基因GUS表达的活性。

关键词: 特征, 特征, 霜冻初终日, 影响, 农业, 山西北部

Abstract:

In previous study, the full length DNA and cDNA of the RdGASA4-like gene had been isolated from Damask rose（Rosa damascena）through homology cloning combined with rapid amplification of cDNA ends(RACE). In order to further understand the role of RdGASA4-like in the regulating network on plant development of gibberellic acid, its 685bp 5’-UTR and partial sequence upstream ATG of RdGASA4-like was obtained by Thermal Asymmetric Interlaced Polymerase Chain Reaction (TAIL-PCR). Based on sequence analysis, it was fused to a uid A gene to construct plant expression vector, named as pBI-GP685. By agrobacterium-mediated method, the explants of petiole with cotyledon of Cabbage were transformed. The transient expression study showed that the obtained promoter of RdGASA4-like was active and can drive the uid A by GUS assay analysis.