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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (7): 107-111.

所属专题： 生物技术

• 农学 农业基础科学 • 上一篇    下一篇

耐低温兼性厌氧淀粉酶产生菌Y89的筛选及酶学特性

李 欣 张丽萍 程辉彩 崔冠慧 付艳菊   

  • 收稿日期:2010-08-13 修回日期:2010-10-10 出版日期:2011-04-05 发布日期:2011-04-05
  • 基金资助:

    国家“十一五”科技支撑项目;河北省科技支撑项目

Isolation of the Psychrotolerant Facultative Anoxic Strain Y89 Amylase and Enzymatic Properties

  • Received:2010-08-13 Revised:2010-10-10 Online:2011-04-05 Published:2011-04-05

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摘要：

从石家庄污水处理厂活性污泥中筛选得到一株兼性产淀粉酶菌株Y89，经形态学、生理生化和16S rDNA序列分析，鉴定为类芽胞杆菌属 (Paenibacillus sp.)。该菌株为耐低温菌，它的最适生长温度为16℃，最适氯化钠浓度5%，最佳pH值为6.0，pH 5.0—9.5范围内均能生长， 培养72-124h为生长稳定期，在40℃进行水浴反应，该菌株酶活较高，为386.13U/ml。

关键词: 甘蔗, 甘蔗, RNA提取, 异硫氰酸胍法

Abstract:

A psychrotolerant strain Y89 producing amylas,which is facultative anoxic , was isolated from activated sludge of sewage treatment plant of ShiJiaZhuang. The morphology, the physiological biochemical characteristics and the 16SrDNA analysis of the strain point out that this organism is a member of the genus Paenibacillus sp.. The strain is psychrotolerant, the optimum growth temperature was 16°C，the NaCl optimum for growth is 5%, the fastest growth occurred within pH6.0, the strain can also grow within a range of 5.0—9.5. The stationary phase of the strain occurred after cultured between72h and 124h ,water bath at 40℃, the amylase displayed maximum activity(386.13U/ml).