欢迎访问《中国农学通报》,

中国农学通报 ›› 2011, Vol. 27 ›› Issue (3): 352-355.

所属专题: 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

Ⅰ型鸭肝炎病毒(DHV-Ⅰ)在鸡胚中的增殖及感染力的测定

马双羽 丁毅 文力正 袁宝 任文陟   

  • 收稿日期:2010-08-25 修回日期:2010-09-27 出版日期:2011-02-05 发布日期:2011-02-05
  • 基金资助:

    吉林省实验动物质量检测中心平台建设

Proliferation and Infectivity of Duck Hepatitis Virus TypeⅠ (DHV-Ⅰ)

  • Received:2010-08-25 Revised:2010-09-27 Online:2011-02-05 Published:2011-02-05

摘要:

摘要:将Ⅰ型鸭肝炎病毒(DHV-Ⅰ)接种9日龄SPF鸡胚尿囊腔(0.2 mL/胚)进行增殖,制备鸡胚成纤维细胞(CEF),并分别运用CELD50法和TCID50法测定DHV的感染力。结果表明,DHV-Ⅰ在SPF鸡胚内成功增殖,致死鸡胚具有典型临床症状和体表特征,所增殖病毒的CELD50和TCID50分别为10-5.6/0.2 mL和10-5.12/0.1 mL。

关键词: 水稻, 水稻, 气候条件, 分析

Abstract:

Abstract: In this study, the proliferation of Duck Hepatitis Virus Type Ⅰ (DHV-Ⅰ) is based on SPF Chick Embryo. Preparation of chicken embryo fibroblasts (CEF), CELD50 and TCID50 are used to determine DHV infectivity. The results showed that DHV successfully proliferated in SPF chicken embryos, Lethal Chick of DHV have typical symptoms and surfaced characteristics, the value of CELD50 is 10-5.6/0.2 mL and TCID50 is 10-5.12/0.1 mL.