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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (23): 259-262.

• 林学 园艺 园林 • 上一篇    下一篇

不同培养条件对金线莲多糖与总生物碱含量的影响

杨翠芹 秦耀国 曾富春 孙歆 文涛   

  • 收稿日期:2010-09-10 修回日期:2010-10-14 出版日期:2010-12-05 发布日期:2010-12-05
  • 基金资助:

    四川省教育厅科研项目

Effects of Different Culture Conditions on Content of Polysaccharide and Total Alkaloids of Anoectochilus formosanus

  • Received:2010-09-10 Revised:2010-10-14 Online:2010-12-05 Published:2010-12-05

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摘要：

To investigate tissue culture conditions to increase certain medicinal ingredients in tissue culture seedlings of Anoectochilus formosanus, using its tissue culture seedlings as the material, set four sucrose concentrations 1%, 2%, 3%, 4% and four treatments of subculture 10, 30, 50, 70 days, and then measured the content of polysaccharide and total alkaloids from its tissue culture seedlings after culturing for some time. The results showed that 4% of sucrose concentration was the most suitable quantity on the accumulation of polysaccharide. Polysaccharide content showed an increase after the first reduction with subculture days. Total alkaloid content decreased with the increase of sucrose concentration, and it was the highest with 1% of sucrose concentration. The content of total alkaloids was the highest after 50 days of subculture.

关键词: 蓖麻, 蓖麻, 杂交种, 比较试验, 产量

Abstract:

To investigate tissue culture conditions to increase certain medicinal ingredients in tissue culture seedlings of Anoectochilus formosanus,using its tissue culture seedlings as the material,set four sucrose concentrations 1％,2%,3%,4％ and four treatments of subculture 10,30,50,70 days,then measured the content of polysaccharide and total alkaloids from its tissue culture seedlings after culturing for some time. The results showed that 4% of sucrose concentration was the most suitable quantity on the accumulation of polysaccharide;Polysaccharide content showed an increase after the first reduction with subculture days. Total alkaloid content decreased with the increase of sucrose concentration,and it was the highest with 1% of sucrose concentration. The content of total alkaloids was the highest after 50d days of subculture.