Abstract: Rose (Rosa rugosa Thunb.) is an important ornamental, but the fast propagation system hasn’t been set up yet. In this paper, we studied the effect of different light intensity on browning of rose initially cultured in vitro. Our results showed that the browning index of rose decreased and the percentage of germination increased after field treatment, especially in the light intensity of 5,333±1,751 Lx. Compared with the light intensity of 5,333±1,751 Lx and 63,078±1,651 Lx, the activity of PPO of rose under natural light intensity showed no significant difference whereas the content of phenolic substances was much higher than those treated with shaded illumination. The co-efficiency analysis demonstrated that there was no obvious relation between the activity of phenol oxidase and content of phenolic substances.