Abstract: This article studied relation between aged-pine’s soil phosphatse activation and soil nutrient of root district from lateral and longitudinal direction in Shenyang east imperial tomb. Soil nutrients determined include organic matter, total nitrogen, available nitrogen, total phosphorus and available phosphorus. The results showed: the most nutrient is absorbed at 4m distance from aged-pine trunk, correlation between soil phosphatse and organic matter, total nitrogen, available nitrogen and total phosphorus are positive marked, but correlation between soil phosphatse and available phosphorus are negative marked; So phosphatse activity could characterize soil P content of aged-pine trunk, as one of the indexes valuing soil fertility in aged-pine trunk.