Cucumber was taken as experimental object in this experiment, better soil supplements and fertilizer were screened by adding different soil supplements into the nursery soil and applying different kinds of fertilizer after harvesting the first-group cucumber, then a series of nursery and fertilizer application technologies which are suitable for the production of organic cucumber in greenhouse of Beijing were formulated. The analysis of this experiment revealed that the height of seedling and the diameter of stem could be obviously promoted by using rough bamboo vinegar (200-fold) and mineral fertilizer 40:1 as soil supplements, applying mineral fertilizer 1500kg/hm2, rough bamboo vinegar 510 kg/hm2 after collecting the first-group cucumber could increase the total output obviously, the increasing rate of production were 35.70% and 30.76%.