Near isogenic lines of restoring gene to V-type CMS of wheat were bred. The mtDNA of cytoplasmic male sterile line, maintainer line, restorer line and F1 hybrid were compared by using ISSR、SRAP and RAPD techniques. The results are as follows: (1) 153 SRAP primers were screened, 139 of them had amplification polymorphisms, 9 primers had fragments that specific existed in cytoplasmic male sterile line, 3 primers had fragments that didn’t exist in cytoplasmic male sterile line. (2) Ninety-two ISSR primers were screened, 6 primers had fragments that specific existed in cytoplasmic male sterile line. (3) Two hundred and eighty-eight RAPD primers were screened, 281 of them had amplification polymorphism, and 4 primers had fragments that specific existed in cytoplasmic male sterile line, 3 primers had fragments that didn’t exist in cytoplasmic male sterile line. SRAP technique is more polymorphism than ISSR, and is more stable than RAPD when analyzing mtDNA.
