Abstract: The purpose of this research is to improve efficiency of tissue culture and transplanting and cultivating of tissue culture derived-plantlets of hundred year old-Chimonanthus praecox(L.)Link. Using annual branch as material, shoot inducing, rapid propagation, rooting and cultivation were studied. The results showed that the 1/2MS(1/2 of macroelements) as basic media was super to Read、WPM、N6, propagation coeifficient was increased and frequency of explants browning was decreased by supplemented with 2.0mg/L 2iP, 0.2mg/L IAA, 0.5 mg/L GA3 and 1.5-5.0mg/L PVP in inducing media, the number of vitreous buds were decreased by reducing hormone concentration, the frequency of rooting was 75% in media containing 0.5mg/L IBA and 0.05mg/L NAA. Transplanting survival rate of tube-plantlets could be improved by hardening plantlets for 1-2 days and maintained humidity for 14 days. The growth of tube-plants was promoted by holding weeds and root-vigor be increased by controlling the level of groundwater.