Abstract:

Abstract：The objectives of the study were to determine the location of entFM gene in genome and the characterization of entFM gene sequence. The probe for entFM gene of WB9 was prepared and hybridized with plasmid DNA and chromosomal DNA of WB9 respectively. The result indicated that entFM gene of WB9 is in the chromosome. The ORF of entFM genes in Bt WB9, TS16, 1072 and Bc 6A1 were cloned and sequenced. Sequence analysis showed that entFM gene of WB9 contains 1281 nucleotide acids, encoding 426 amino acids.The predicted amino acid sequences of entFM gene of WB9, TS16, 1072 and 6A1 were compared with Bc Cx5, the result showed that there are high homologous among peptides of WB9,TS16,1072 and Cx5,the main differences between WB9 and Cx5 are that 4 amino acids (Gln-Thr-Gln-Thr) are deleted between 39th and 42th in WB9 and 4 different amino acids(SWDK) are added between 96th and 99th in Bc Cx5.