Current issue

• 2009 Vol.25 No.19 Published:05 October 2009

  Molecular Basis of Pathogenesis of FMDV 5’Untranslated Region 2009, 25 (19):  1-5.  Abstract + ( 1511 )   PDF ( 537646KB )   ( 932 ) This paper expatiated the structure and function of the Foot-and-Mouth Disease Virus(FMDV) 5’Untranslated Region and summarized the recent advances in study of the pathogenesis and effects of the FMDV 5’Untranslated Region on its host cells．The molecular basis of pathogenesis of FMDV 5’Untranslated Region have been analyzed in this paper and considered that the genome structure of the FMDV 5’Untranslated Region are essential for replication and production of disease,which would provide a valuable theoretical reference for further studying on replication,virulence and its determinants,cell and host tropism and pathogenesis of FMDV.

  Study on hair follicle structure during anagen of Huacaya alpacas (Lama pacos) 2009, 25 (19):  6-9.  Abstract + ( 1420 )   PDF ( 6468328KB )   ( 612 ) Skin from Huacaya alpacas was used to study the structure of during prosperous and fiber, so as to provide some effective scientific bases to appraise germplasm character. The results showed that the hair follicle structure of alpacas was in the same manner compared with other animals, which was composed of hair bulb, connective tissue sheath, internal root sheath, external root sheath and hair shaft.; diameter of hair follicle during anagen was increased gradually, extreme was hair bulb, and hair papilla formed if hair bulb emboled. The alpaca’s hair fiber squamae arranged porous, and there were many hair follicles, which were severed two clusters by the connective tissue.

  Effect of Selenium-enriched Beer Yeast on the Growth Performance and the Antioxidant Capacity in Broiler 2009, 25 (19):  10-13.  Abstract + ( 1547 )   PDF ( 536124KB )   ( 735 ) A total of sixty broilers aged 1 day were randomly divided into five groups. The first group was control group fed with basal diet, the second, third and forth were the experimental groups fed with diets containing 0.2, 0.5, and 1.0 mg?kg-1 selenium by supplying the selenium-enriched beer yeast, the fifth group fed with diets containing beer yeast. The body weight, the whole-blood glutathione pereoxidase (GSH-Px) activity, plasma superoxide dismutase (SOD) activity and total oxidation capacity (T-AOC) value were detected on days 14 and 28. The results showed that body weight of these groups was not different on days 14 and 21, but on days 28, there was a significant difference between the 2nd, 3rd group and the 1st group. The whole-blood GSH-Px activity, plasma SOD activity and T-AOC value in the experimental groups were significantly or more significantly higher than those of the 1st and 5th group. It is concluded that the selenium-enriched beer yeast, which could increase the activity of GSH-Px and SOD, T-AOC value, is a better source of Se for broiler.

  Sceening, identification of the antagonistic bacteria against Verticillium dahliae Kleb and characteristic analyses of antagonistic substance 2009, 25 (19):  14-19.  Abstract + ( 1493 )   PDF ( 722772KB )   ( 787 ) Cotton Verticillium wilt, which is caused by Verticillium dahliae kleb is a worldwide disastrous plant vascular disease. Bacteria strain 12-51 with a rather higher antagonistic activity was obtained after primary and secondary screening. The morphology characteristics, physiological and biochemical properties and 16S rDNA sequence of this strain were studied. The similarity of the 16S rDNA sequences between bacteria strain 12-51 and Bacillus velezensis were up to 99.74%. So, the strain 12-51 was finally identified as Bacillus velezensis. It was determined preliminarily that the extracts were proteins by analyzing the antifungal activity of the extracted substance through organic solvent extraction and salting-out method against Verticillium dahliae Kleb. The results showed that the component of 60% extracted substance had the biggest inhibition zone and the antifungal protein was not sensitive to the heat and pH value. The results laid the foundation for the biological control of Cotton Verticillium wilt.

  Intergratation,Classification and Facticity Analysis of QTLs for Root Morphological Traits in Rice 2009, 25 (19):  20-25.  Abstract + ( 1661 )   PDF ( 1024196KB )   ( 833 ) To improve the utility efficiency of molecular marker assistant selection for drought-resistance traits of rice roots, by using the saturated molecular marked genetic linkage map, we integrated published QTLs for drought-resistance traits of rice roots and analysed the contribution rate, characters of distribution and facticity. The results are as below: Eleven main effect QTLs were determined by the analysis of contribution rate; Eleven QTL regions on chromosome 1,2,3,5,11 were confermed to have pleiotrophic effect according to location of QTLs on each chromosome; Thirty five “real-QTLs” and three “hot-QTLs” are found through the facticity analysis of these QTLs. These main effect QTLs ，pleiotrophic QTLs and “hot-QTLs”can be treated as a significant candidate area for molecular breeding of drought-resistance traits of rice roots.

  Bioinformatic analysis of plant cell wall degrading enzymes in the genomes of four different fugi 2009, 25 (19):  26-31.  Abstract + ( 1723 )   PDF ( 701127KB )   ( 999 ) This paper presents a comprehensive study on identifying the number of genes encoding different classes of plant cell wall degrading enzymes in the genomes of pathogenic and nonpathogenic fungi. Plant pathogenic fungi secrete extracellular enzymes that are able to degrade the plant cell walls of their host plants. These plant cell wall degrading enzymes are so necessary for penetration of the cell wall barriers, as well as for generation of simple molecules that can be assimilated for growth. Initially, BLAST was exerted to compare pathogenic (M.grisea, F.graminearum) and non?pathogenic fungi (N.crassa, A.nidulans) and the top hits of them were acquired. Then SignalP were performed to verify whether these PCWDEs would be secreted by the fungi.Consequently, we conclude that some significant biological differences of PCWDEs can be discovered between pathogenic and nonpathogenic fungi.

  Comparison of 5 Different Extraction Methods for Citrus aurantium DNA 2009, 25 (19):  32-36.  Abstract + ( 1630 )   PDF ( 919311KB )   ( 810 ) Five methods namely improved CTAB method, CTAB-silica extraction method, SDS method, low pH extraction method with high salt and Zhou shiliang method were adopted to extract genome DNA from the leaves of Citrus aurantium. And three methods including ultraviolet spectrophotometer, agarose gel electrophoresis and SSR-PCR amplification were used to detect extracted sample DNA. The results indicate that SDS method is better than other 4 DNA extraction methods, DNA concentration is highest（Its average is 900 ng/ul）and DNA purity is best（Its average is 1.90）, and SSR-PCR amplification results with UV-spectrophotometer and gel electrophoresis test results are basically the same. So SDS method is the best DNA extraction method for Citrus aurantium, and DNA quality suitable for molecular detection experiment.

  Optimization of ISSR-PCR Reaction System in Wampee 2009, 25 (19):  37-41.  Abstract + ( 1296 )   PDF ( 1071635KB )   ( 787 ) Abstract：The factors influencing ISSR-PCR to analyze the genetic divergence in Wampee were investigated by a single factor test and orthogonal design experiment.The results showed that a better amplification system of ISSR was obtained with the reation system comtaining 30ng template DNA,1.5U Taq DNA polymerase,0.4μmol/L primers,0.3mmol/L dNTPs in the total volume of 20μL.The optimized annealing temperature was 52.4℃ for primer (gA)8C.

  Advancement on mining and utilization of elite genes in Oryza officinalis Wall 2009, 25 (19):  42-45.  Abstract + ( 1637 )   PDF ( 533702KB )   ( 832 ) Oryza officinalis Wall accumulated abundant genetic diversity and contains a large number of important beneficial genes. The indistinct molecular genetics background of Oryza officinalis Wall and the low efficiency of conventional breeding were two obstacles on the utilization of Oryza officinalis Wall. By using transgenic technology to transfer large fragment to cultivated rice to buiding gene insertion mutant libraries and transformation-competent large fragment libraries of wild rice, these problem were resolved. In this paper the auther summaried the research of excellent genes in Oryza officinalis Wall and utilization of those genes

  The relation between compatibility and water-solubility sugar of tobacco 2009, 25 (19):  46-49.  Abstract + ( 1293 )   PDF ( 596062KB )   ( 687 ) In order to investigate the relations of the compatibility between different tobaccos to the water dissoluble sugar content in the same,13 kinds of middle leaves from Henan tobacco growing areas were tested in pairs and the compatibility of each pair was panel evaluated and scored so as to study the compatibility between the paired tobacco and its relation to their water dissoluble sugar contents. The results showed that the average between the water dissoluble sugar contents of the paired leaves affected not only their general compatibility but also the compatibilities between their chroma and their combustibility; and the difference affected their general compatibility. The compatibility of tobacco was better when the average is about 25.0～30.0%or difference was lower than 7%.

  Breeding Value of the Wheat Corner Stone Parent Yumai 2 2009, 25 (19):  50-54.  Abstract + ( 1580 )   PDF ( 518287KB )   ( 957 ) In 183 registered wheat cultivars of Henan Province, 75 cultivars have consanquinity of Yumai 2. Yumai 2 is the most widely utilized corner stone parent in wheat breeding of Henan Province. The coancestry coefficient of Yumai 2 with its 75 derived cultivars were analyzed.The results showed that coancestry coefficient of the 75 wheat cultivars with Yumai 2 varied from 0.0500 to 0.6250.The total coancestry coefficient was 20.9401, which accounted for 27.87% in 75 wheat cultivars and 11.42% in 183 registered wheat cultivars of Henan province.Yumai 2 had very high value in use, and this would continue in coming years. The study of the breeding value of Yumai 2 was of great significance for utilizing wheat germplasm effectively, maintaining the genetic diversity of wheat lines, selecting the hybrid parents properly, and succeeding in breeding a good new genotype.

  Study on Improvement Effect of CIMMYT Germaplasm to the inbreds of a corn hybrid Dongnong250 2009, 25 (19):  55-58.  Abstract + ( 1299 )   PDF ( 606079KB )   ( 612 ) CIMMYT germaplasm Pob46 and Pob45 were introduced respectively the male inbred lines Ji846 and female inbred lines 444. The improvement effect of yield and other agronomic characters was studied. It showed that in the hybrids crossed by the improved lines of Ji846 and 444, plant height and ear height were decrease, lodging resistance character was enhanced, ear-kernel weight was increased,. The yield of these crosses decreased commonly, only individual crosses increased; The improvement effect of some characters was different in these crosses, such as the first class branch number of tassel and kernel number per row.

  Detection of seven kinds of Organophosphorus pesticides Residues in Vegetable by Gas Chromatography 2009, 25 (19):  59-61.  Abstract + ( 1638 )   PDF ( 526904KB )   ( 728 ) A method for determ ination seven kinds of organophosphorus pesticides in vegetables were studied with capillary post and Gas Chromatography 2010 phase with FPD detection. The recoveries of these organophosphorus pesticides are between the range of 83.5%～112.3% , and the opposite and standard deviation are between the range of 0.99 %～3.73 %.The results of this test had met the requirement of national method of pesticide residue analysis. The method introduced here is simple, feasible and reliable.

  Changes of Anti-oxidant Enzymes Activities during Florescence and Flower Senescence of Navel Orange jy 2009, 25 (19):  62-64.  Abstract + ( 1416 )   PDF ( 590342KB )   ( 870 ) Florescence and flower Senescence are complicated physiological and biochemical processes. Navel orange was taken as experiment material to investigate the changes of anti-oxidant activities during florescence and flower senescence. The results showed that MDA content accumulated and the activities of SOD, CAT, GPX first increased then decreased, but APX decreased during florescence and flower senescence. Therefore, one of important reasons for florescence and senescence of navel orange is the disorder of metabolism induced by the accumulation of active oxygen species.

  Study on the Development Situation and the countermeasure of the Follow-up Industrial Development after Slop Land Conversion Program in Zhengzhou City 2009, 25 (19):  65-68.  Abstract + ( 1647 )   PDF ( 489950KB )   ( 725 ) Beginning with the importance of developing the following-up industry after Slop Land Conversion Program, the article discussed the present situation of the following-up industry in zhengzhou, and analyzed the existing problems, then proposed the development countermeasure and the pattern according with the actual situation in Zhengzhou city. This provided the reference and model for consolidating the achievement of the Slop Land Conversion Program and promoting the development of the following-up industry in zhengzhou.

  Analysis the Environmental condition of Implementing for Converting Farmland into Forest in Donglan County 2009, 25 (19):  69-72.  Abstract + ( 1279 )   PDF ( 526540KB )   ( 636 ) Abstract [Objective] Following management control provides the theory basis for converting farmland into forest in Donglan County.[Method] Adopt the method that Consult material and the sample of the typical sampling for samples survey,It have been completed the investigation that for the natural environment condition and the social environment condition and the forestry working condition and land resource condition in Donglan County.[Result]The Environmental condition of Donglan County Suits the returning farmland to forest project the implementation.[Conclusion] Implements Returning farmland to forest's suitable tree seed mainly to have Castanea mollissima, Illicium verum and Camptotheca acuminate in Donglan County.

  The research on anatomical structure of stem from Cornus officinalis 2009, 25 (19):  73-75.  Abstract + ( 1580 )   PDF ( 7389561KB )   ( 595 ) The anatomical structures of stem from Cornus officinalis were studied by the method of paraffin sectioning and optical microscopic observation. Results show that the primary structure consisted of epidermis，cortex and central cylinder from the outer to inner；epidermic cells are arranged closely，and the horny layers of the epidermic are depth of 1-1.5 ?m about；There are many collenchymas in cortex which are thriving；Pith is developed and has an obvious belt. The secondary structure included periderm and secondary vascular tissue from the outer to inner，there are indistinct between spring wood and autumn，and the vessels are scattered in secondary xylem. The features of primary structure and secondary structure adapted to the xenomorphic.

  The Effect of Different Form Nitrogen Addition on Decomposition of Schima superba and Pinus massoniana Leaf Litter 2009, 25 (19):  76-81.  Abstract + ( 1399 )   PDF ( 621972KB )   ( 611 ) Abstract: The effect of different form nitrogen addition on decomposition of Schima superba and Pinus massoniana leaf litter from mid-subtropical zone were studied using a self-designed lysimeter. The results showed that: decomposition rates would all be obviously accelerated by NH4Cl and NaNO3 adding, and added more -N and -N liquor, S. superba leaves decompose more quickly. But compared with the effects of same quantity of distilled water, the accelerating effects of -N and -N could very significantly appear till added 300 ml liquor. To some extend the decomposition rates of P. massoniana needles also would be accelerated by adding NH4Cl and NaNO3, but compared with CK the effects of a certain quantities of distilled water were stronger than -N and -N, and in the three treatments olny when add 200 ml NaNO3 liquor could more obviously accelerated P. massoniana needles decomposition rates than distilled water. On the wholel the effects of addition N on P. massoniana needles decompose were not as stronger as on S. superba leaves.

  Genetic variation Analyse of drought-resistance traits with F2:3 family of wheat 2009, 25 (19):  82-86.  Abstract + ( 1635 )   PDF ( 666631KB )   ( 803 ) Abstract: Four hundred and two F2:3 families, developed from the cross between drought -sensitive variety Yan Zhan 2 and drought-resistance wheat variety Shi 4185, were evaluated for its starch content in the replicated experiments under irrigated and dry land two environments. Based on the analysis of the F2:3 family, the genetic behaviors of 9 quantitative traits including plant height, spike length, in-ternodal length below spike , flag leaf width and area ,fertilized spikelet per spike,numbers of spike per plant, number of spike grains and weight of spike grains . The results revealed that there were obvious segregations for all traits among F2:3 family, under the condition of irrigated and dry land. All traits showed normal distributions.This reflected that this F2:3 family was suitable for QTL mapping for all plant traits. However, there were variations in F2:3 family for almost all traits.

  Comparison of Maize densities With Cultivation Technique of Partial Ridge Permeability 2009, 25 (19):  87-89.  Abstract + ( 1229 )   PDF ( 505362KB )   ( 545 ) In view of maize cultivation techniques partial ridge permeability characteristics, to achieve the requirements of maize production, compared to the density test. Selected for partial ridge permeability cultivars on the Xinxin 1st in Baiquan， best density is 4760 / 667 ㎡， output reached 728.48 kg / 667 ㎡.

  Effects under combined treatment of Manganese and EDTA- Fe on qualities of Mengshan tea in Western Sichuan 2009, 25 (19):  90-94.  Abstract + ( 1530 )   PDF ( 1682111KB )   ( 639 ) Manganese and EDTA-Fe in the soil both are the beneficial elements for plant growth. Effects under combined treatment of Manganese and EDTA-Fe on quality of Mengshan tea were studied using D- Saturating Optimum Design. The results showed that, when Manganese concentration of treating was 0.12g/kg and EDTA-Fe concentration was 0.03g/kg, the interactive effects of the tea polyphones and Caffeine were highest, and the average contents were 24.78% and 2.96%. when Manganese concentration of treating was0.12g/kg and EDTA-Fe concentration was 0.24g/kg , the interactive effects of the amino acid and the soluble sugar were highest and reached the theoretical maximum, and the average contents were 2.19% and 2.65%.

  Study on Allelopathy of Medicago sativa Stem Aqueous extracts 2009, 25 (19):  95-99.  Abstract + ( 1619 )   PDF ( 786415KB )   ( 630 ) In the experiment , Allelopathy of Medicago sativa on Dactylis glomerata L was studied through stem leaf water extract in seedling stage. It turned out that allelopathy of Medicago sativa on Dactylis glomerata L was evident. Eurek and Baralfa obviously inhibited germination and seeding growth ,but promoted leaf dry weight. Sanditi and Sadie was not significant on seeding length of Dactylis glomerata L.,but it can inhibited seeding length.Moreover, The results also show that the allelopathic inhibition rate increased with intense concentration of the water extracts.

  Effect of Casuarina equisetifolia autotoxicity on endogerous hormones 2009, 25 (19):  100-103.  Abstract + ( 1672 )   PDF ( 646087KB )   ( 678 ) Effect of autotoxic substance of Casuarina seedling on endogerous hormones were studied using the method of nutritional culture and regarding root water extract of 15-year casuarina. The results indicated that the content of the growth promoting hormones in leaves and roots of casuarina IAA、GR、iPAs and ZRs decreased under autotoxic substances treatment and the content of decreasing was strengthened with the increasing of concentration of autotoxic substances. And the balance of endogenous hormones were broken and the content of ABA increased as well as ABA/IAA,ABA/GA,ABA/iPAs and ABA/ZRs. Under autotoxic substances treatment, the increment and biomass of roots, items and leaves of casuarina has close relation to the reducing of content of IAA,GR,iPAs and ZRs, increasing of content of ABA and the damage of the balance of endogenous hormones.

  Effect of Shading on Diurnal Variation of Photosynthetic Characteristics of Vihurnum opulus L. song 2009, 25 (19):  104-107.  Abstract + ( 1569 )   PDF ( 596298KB )   ( 641 ) In order to understand the tolerance to shade of Vihurnum opulus L, the diurnal variation of photosynthetic characteristics and its relationship to the environmental factors of Vihurnum opulus L. under different shading treatment were studied with the Licor-6400 portable photosynthetic system. The results showed as following: (1) The diurnal variation of net photosynthetic rate (Pn) of Vihurnum opulus L. showed double-peak curve under the 100% light and 60% light, and showed obviously midday depression, however, under 20% light, the diurnal variation of Pn showed single-peak curve. (2) The Vihurnum opulus L’s controlling factor of photosynthetic rate was non-stomatal limitation. (3) Under 100% light and 60% light, the first factor affecting Pn was stomatal conductance(Gs); Under 20% light, the first factor affecting Pn was Transpiration rate(Tr).(4) The proper shade had not strong effect on the photosynthesis of Vihurnum opulus L. To strengthen the shade, the photosynthesis rate decreased significantly. The photosynthetic characteristics’ ability of about three treatments were 100% light＞60% light＞20% light. Vihurnum opulus L could be planted in the shadow.

  Research Progress on Enzymatic Antioxidant System Response to Stress in Cotton 2009, 25 (19):  108-112.  Abstract + ( 1565 )   PDF ( 504013KB )   ( 797 ) Stress had a serious effect on yield and quality of cotton . It could lead to reactive oxygen species (ROS) increasing in cotton plant, and enzymatic antioxidant system which could scavenge active oxygen, played an important role in adverse resistance physiology during cotton growth. In this paper, on the basis of a brief introduction of cotton enzymatic antioxidant system, the research progress on enzymatic antioxidant system response to different stresses, such as drought, salt injury, verticillium and so on, in recent years were reviewed. The problems and prospects on enzymatic antioxidant system response to stress in cotton were put forward.

  Differences in Alkaloid Contents Among Different Leaf Portions for Flue-cured Tobacco with Full Aroma Style 2009, 25 (19):  113-115.  Abstract + ( 1438 )   PDF ( 1061128KB )   ( 666 )

  Discussion of Effect of Nitrogen Application on Biomass and Production of Y Liangyou 1 2009, 25 (19):  116-122.  Abstract + ( 1482 )   PDF ( 784587KB )   ( 734 ) 【OBJECTIVE】Effect of different N-fertilizer levels and different N-fertilizer ratio on actual yield and yield components of super hybrid rice was studied 【METHOD】Two field experiment about different N-fertilizer levels and different N-fertilizer ratio were designed through super rice Y liangyou No?1 was experiment material 【RESULTS】 It was significantly secondary correlated between yield, ear length, grain number per panicle and nitrogen application rate (p < 0.01) and linear correlated between panicle number per ha and nitrogen application rate (p<0.05). In the amount 180 kg?hm-2 of nitrogen application, the production was reached 11649 kg?hm-2 and 11624 kg?hm-2 for 60-40 of base-panicle N fertilization and 10-50-25-15 of base-tillering-panicle-granular N fertilization respectively..Rice biomass was increased with N-rate increasing in different period; The effect of biomass promoted by N-rate was especially obviously with the progress of rice growth; Rice biomass change trends were similarly at different stage in different N-rate under same base-panicle (80-20) N-ratio. Yield of super hybrid rice was generally higher when rice biomass increased great in late period. 【CONCLUSION】252 kg?hm-2 was the N fertilizer rate when the yoeld of Y liangyou No. was highest. In the amount 180 kg.hm-2 of nitrogen application, the production was reached 11649 kg.hm-2 and 11624 kg?hm-2 for 60-40 of base-panicle N fertilization and 10-50-25-15 of base-tillering-panicle-granular N fertilization respectively.

  Effects of Single Fertilizers Application on Yields and Environment of Spring Maize Production 2009, 25 (19):  123-128.  Abstract + ( 1430 )   PDF ( 935271KB )   ( 841 ) Field experiments were conducted in higher and lower fertility soil to evaluate effects on maize grain yield, soil residual NO3-N and N apparent losses of three nitrogen management strategies, conventional N splits by farmer, one shot dose fertilization by farmer and single basal application with polymer-coated urea (PCU) as partial N source. The results of two experiment sites showed that there was no significant difference on yields of different N management. Increase of 0-90cm soil residual NO3-N in Xinlicheng site of control, conventional fertilization, one shot does and single application with PCU treatments were -17.5，104.5，55.7，29.3kg N/hm2, and -44.3，-12.8，-57.2，-51.7 kg N/hm2 for higher fertility soil (Buhai site), respectively. The apparent N losses of whole stage were -83，-12，22 kg N/hm2 for lower fertility soil, and 172，119，157 kg N/hm2 for higher soil fertility soil. It indicated that less residual NO3-N increase with treatment of single application with PCU compared with other two strategies, and more apparent N losses resulted from rising of N rate.

  Amino acid organic Compound fertilizer effect on growth and development, output value and Qualities of tobacco. 2009, 25 (19):  129-133.  Abstract + ( 1318 )   PDF ( 532459KB )   ( 604 ) Amino acid organic compound fertilizer was applied on tobacco in Kunming, Wenshan, Puer, Linchang , The results indicated that amino acid organic compound fertilizer can enhance tobacco early growth and quick tiller, output value and yield of per mu, the mean price, the ratio of middle class and superior tobacco leaves, and the growth and development of tobacco was in advance. By applying amino acid organic compound fertilizer, the nitrogen and alkaloid content in the tobacco leaves increased, while the total and reducing sugar content decreased. there were more aroma quality and irritation with lessen offensive odor, with no significant differences in the others content of leaves.

  Function Evolvement of Straw and Effect of Effective Microorganisms 2009, 25 (19):  134-138.  Abstract + ( 1377 )   PDF ( 535242KB )   ( 872 ) Through analyzed the function evolvement of straw, the most straw use patterns was return fields at present, which can protect ecological environment in agricultural system. Whereas, with many straw cover soil surfaces, there are many adverse influences, for example, it can baffle Mechanical seeding and improve crop development. Many research indicated that effective microorganisms improving those adverse ecological impacts at some extent. Our experiments also proved that effective microorganisms increased crop yields about 20%, result indicate that effective microorganisms can accelerate crop growth.

  Study on Nutrient Release Rate of Some Organic Polymer Coating Controlled-release Fertilizers ZHAO ZHAO Xiu-Fen 2009, 25 (19):  139-141.  Abstract + ( 1252 )   PDF ( 604143KB )   ( 707 ) Three kinds of controlled-release fertilizers, Osmocote, Meister and the sample of China, coated by organic polymer, were used in this study. The nutrient release rate of fertilizers were tested and compared in water. The effect of temperature on the rate of fertilizers’nutrient release was discussed. Results indicated that merely labeling single element released period was illogical. Results were evaluated comprehensively, and the key of the control of fertilizers’nutrient release was selecting proper coating substance.

  Effects of different concentration of NO3- on the ChlorophyⅡ Fluorescence sweet cherry under waterlogging 2009, 25 (19):  142-146.  Abstract + ( 1558 )   PDF ( 591435KB )   ( 991 ) Effect of NO3- on the ChlorophyⅡ Fluorescence of the meizhao/colt under waterlogging were studied .The results show that with the increase of waterlogging, (Fv/Fm )、 Fv/Fo and (qP) had the trend of decrease –increase-decrease. Exogenously applied NO3- alleviated the decrease of Fv/Fm 、 Fv/Fo and qP. qN on The treatment of NO3- were higher than CK. The light energy conversion efficiency, and potential activity of photosynthetic reaction center and the non-photochemical dissipation of excess light enemy, which resisted the damage on photosynthetic apparatus of environmental stress, were improved.

  Effect of organic materials on available nutrients and microbial numbers of grape replant soil 2009, 25 (19):  147-151.  Abstract + ( 1716 )   PDF ( 602749KB )   ( 651 ) Effect of different proportions of straw and corn stalk on available nutrients and microbial numbers in grape replant soil was studied by pot experiment. The results showed available N content of replant soil decreased after straw and corn stalk application, however, available P and available K contents increased in different degree. The numbers of bacteria, actinomycetes and fungi in replant soil increased after straw and corn stalk application. The ratio of bacteria and fungi increased, and that of actinomycetes decreased in straw treatment. The ratio of fungi decreased in corn stalk treatment, and the ratio decreased as the rise of applied amount of corn stalk . Except 99.5% replant soil＋0.5% corn stalk treatment ,the ratio of bacteria increased and that of actinomycetes decreased.

  A Study on City Landscape Design of Public Open Space Based on Dotted-like and Linear System 2009, 25 (19):  152-156.  Abstract + ( 1414 )   PDF ( 548400KB )   ( 950 ) The development of the landscape is going through a course of civilians and mass. Modern Landscape should be shown to all the members of the public equally. The essence of landscape places is people's living area. It should be consistent with ecological requirements, basic public leisure demands and cultural needs. Its practice is behaved in landscape design, which aims to create public open space for citizen. This thesis starts with the concept of landscape and popular landscape development. Then urban public landscape designs are discussed from dotted-like and greenway linear space perspective. It can be used for reference to create more civilian and ecological urban landscape.

  The Introduction Observation and Ornameatal Application of Chaenomeles in Zhejiang 2009, 25 (19):  157-160.  Abstract + ( 1336 )   PDF ( 497155KB )   ( 662 ) In order to enrich the ornamental plant resources in southern region，the experimental introduction of chaenomeles was studied in Zhejiang Jiaxing. Under the conditions of general management，the results showed that the growth performance of such varieties as ‘Fuse’ was normal. They were the excellent ornamental plants，could be used in yard，street，road and garden landscape．And was also used in the production of high-grade art bonsai．Combined with their ornamental biological characteristics，means of their application were discussed．

  Effect of 60Co-γ Ray radiation on the Germination Rate and Growth of Tagetes erecta 2009, 25 (19):  161-163.  Abstract + ( 1342 )   The effect of five different radiation dosages of 60Co-γ ray on the Tagetes erecta was studied. The result showed that 80Gy and 160Gy were suitable for the Tagetes erecta seeds based on the influence of germination rate and the length of bud and root. 20Gy and 320Gy 60Co-γ ray radiation can restrict the germination rate and the growth of bud and root. But 60Co-γ ray radiation restricted the growth and kaihua.The stem height and inflorescence diamet of Tagetes erecta were smaller than comparison.

  A discussion on the cold injury grade of Longan 2009, 25 (19):  164-166.  Abstract + ( 1217 )   PDF ( 415902KB )   ( 568 ) The present paper summarized the issues of cold injury evaluation on Longyan and analyzed the factors relation to the injury grades when the standard grades are constituted. Based on this, a new evaluation standard for cold injury grade in Longan was suggested as well as the necessary field measures according to different injury grades.

  Research on Coastal Rural Building Settlement Landscape Instruction in Shandong peninsula——A Case Study of Lidao Town in Rongcheng 2009, 25 (19):  170-173.  Abstract + ( 1411 )   PDF ( 546396KB )   ( 673 ) The coastal rural building settlement in Shandong peninsula has been present a series of urbanization problems. Through analyzing these problems, three principles for renewing it are offered: dynamic protection、cultural inheritance、sustainability. This article takes Lidao town in Rongcheng city as the case to study on Optimization of the rural building settlement. To protect and utilize the coastal rural building settlement in Shandong peninsula, there are two countermeasures: renewal of rural building settlement、protection concept guiding for the rural residents.

  Effects of extreme higher temperature on germination and seedling growth of Celosiae Cristatae 2009, 25 (19):  174-177.  Abstract + ( 1774 )   PDF ( 6288092KB )   ( 651 ) This paper discussed the seed germination of Celosiae Cristatae could endure the extreme higher temperature. After treated with extreme higher temperature for Celosiae Cristatae seeds, Celosiae Cristatae seeds germinated and growed, came to flower, some changes were observed in the history of the entire growth. After the test, The results showed that after extreme higher temperature and at different time in different treated for Celosiae Cristatae seeds, resistance of seedlings declined in varying degrees, seedlings were susceptible to damping-off. The effect of extreme higher temperature treatment on the Celosiae Cristatae flowers color and pattern of flowers were little, increasing color of variation could not be found out. After extreme higher temperature processdure on Celosiae Cristatae seeds, seedling growth became significantly slower than that of the comparision.

  Development Strategy of Pomology Science and Technology in Shijiazhuang City 2009, 25 (19):  178-181.  Abstract + ( 1133 )   PDF ( 503993KB )   ( 638 ) Development Strategy of Pomology Science and Technology in Shijiazhuang City （1.College of Management; Hebei University of Technology; Tianjin 300130；2.Hebei Academy of Agricultural&Forestry Sciences; Shijiazhuang 050051） Abstract: In this paper, the author analyzed the present situation and the tendency of fruit tree technology development in Shijiazhuang, analyzed the progress status and problems of Shijiazhuang fruit tree technological progress. From the standpoint of fruit tree strategic development in Shijiazhuang, the author clearly put forward the guiding principle of fruit tree development of Shijiazhuang, determined the priority filed and key technologies for future development of agriculture science and technology. The author also proposed the strategies for development of agriculture science and technology such as: liberating the thinking, renewing the concepts, accelerating the reform and innovation, optimizing production system, adjusting cropping structure, increasing investment to science and technology, improving fruit quality, optimizing the allocation of resources, and strengthening government support and regulation, etc.

  Genetic diversities among 10 representative Camellia cultivars based on DNA molecular marker 2009, 25 (19):  182-185.  Abstract + ( 1807 )   PDF ( 1154816KB )   ( 739 ) Abstract: Genetic diversities among 10 representative camellia cultivars in Wenzhou were investigated by RAPD technology（random amplification polymorphic DNA）. The results indicated that 105 bands were produced with 15 random primers, and the genetically polymorphic bands numbered 79 accounting for 77.54%. Genetic distance analysis represented genetic distances among 10 samples ranged from 0.2236 to 0.4777 with the biggest value between samples DaZhuS and Gay Chielftain and the lowest between JinPanLZ and Gay Chielftain. Dendrogram by UPGMA software disclosed that 3 groups were produced at the similarity coefficient of 0.62, with sample Benten-kagura and DianXue isolated and the rest clustered together.

  Effect of soil humidity on germination of Sphacelotheca reiliana teliospore and head smut resistance identification for new maize varieties in Yunnan Province 2009, 25 (19):  186-189.  Abstract + ( 1520 )   Teliospores of Sphacelotheca reiliana could germinate very well at 22℃ and between 20％～25％soil humidity with germinating maize seed, however, they could not without the seed. In 2007 and 2008, 302 new maize varieties from Yunnan Province were identified for their head smut resistance by the inoculation with soil carrying 1% teliospore in two disease nurseries. Among them, 65, 118, 27, 86 and 6 varieties were highly resistant, resistant, moderately resistant, susceptible and highly susceptible to the disease with 21.45%, 38.94%, 8.91%, 28.38% and 1.98%, respectively. It was suggested that only should the resistant and highly resistant varieties be extended in severe disease areas based on the coincidence of incidence with loss rate.

  Symptomology of Tomato spotted wilt virus on Tobacco 2009, 25 (19):  190-193.  Abstract + ( 1508 )   PDF ( 2742335KB )   ( 831 ) This research (paper) systematically described symptoms after Tomato spotted wilt virus (TSWV) infacted tobacco based on the tobacco field survey in North Carolina in 2006. These symptoms included leaf deformation, vein banding, vein necrosis, necrosis spots, concentric rings, rugosity, and stunting growth. By manually mechanical inoculating tobacco plants, the plants were systematically infected and chlorotic spots, necrotic spots, concentric rings, and rugosity symptoms were induced. Finally, the plants died in 4-5 weeks. After comparing two hosts, it was found that the symptoms from two hosts infected with TSWV were similar, but with exceptions.

  Virulence of Verticillium lecanii MZ041024 to Larvae and Pupae of Lycoriella pleuroti 2009, 25 (19):  194-196.  Abstract + ( 1882 )   PDF ( 446490KB )   ( 632 ) Abstract : It was determined the virulence of Verticillium lecanii MZ041024 on Larvae and pupae of Lycoriella pleuroti. The bioassay results indicated that insecticidal activity of ethyl acetate was higher than that of others, their mortality rate of larvae and pupae respectively were 91.79% and 88.94%, with LC50 values of 1.745×105 spores /ml and 4.723×105 spores /ml ; LT50 was respectively3.51d and 3.78d, against Larvae and pupae of Lycoriella pleuroti , respectively.

  Preliminary Study on Gentian spot blight Histopathology 2009, 25 (19):  197-199.  Abstract + ( 1287 )   PDF ( 1161624KB )   ( 620 ) Cultivate plants flowering gentian was selected to inoculate gentian spot blight pathogen, and then was to placed inside the insulation moisture greenhouse to cultivate every certain period of time, sampling, fixed, film, microscope observation of disease and pathogen morphology Change. The results showed that pathogen conidia germination germ tube after the invasion from pore to complete the required invasive 12h ~ 48h, the 10d show symptoms around, 16d browser ripe conidia; host bacteria cause symptoms of infection, the mycelium from the Under surface of leaf across the mesophyll cells, in thin-walled small positive inter-leaf assembly, and finally the formation of conidia, and conidia generated subepidermal parenchyma cells in between, a certain randomness.

  Effect of the Host Plants on Activity of 4 Detoxifying Enzymes in Beet Armyworm, Spodoptera exigua 2009, 25 (19):  203-205.  Abstract + ( 1660 )   PDF ( 502718KB )   ( 810 ) The effect of host plants，including alfalfa，amaranth，lettuce，cabbage，corn，on activity of acid phospholipase，alkaline phospholipase，glutathione-s-transferase and acetylcho linesterase in beet army，Spodoptera exigua was studied. Results showed that significant activity changes of acid phospholipase，alkaline phospholipase，glutathione-s-transferase and acetylcho linesterase were found in Spodoptera exigua larvae feeding on different host plants. The others have marked difference between one another except no difference on activity of basic phospholipase between feeding on amaranth and feeding on alfalfa，cabbage.

  Application of EXCEL in Calculating of Toxicity for Pesticide Bioassay 2009, 25 (19):  206-208.  Abstract + ( 1666 )   PDF ( 500849KB )   ( 1461 ) Based on the principle of Probit Analysis, the Data Processing System For Pesticide Bioassay was established with EXCEL. After the user input the insecticide dosages(or concentrations), the trial number of overall insect and dead insects, the procedure will calculate the toxicological regression line(LD-P), median lethal dosage(LD50), 95% fiducial limit of LD50, relation coefficient(r), and chi square test(χ2) in the insecticide toxicological test quickly and accurately, moreover, also can calculate the chi square test and draw the toxicological line.

  Infectivity of Gaeumannomyces graminis var. tritici on the seminal and secondary roots of wheat 2009, 25 (19):  209-212.  Abstract + ( 1684 )   PDF ( 538128KB )   ( 622 ) The infectivity of the take-all causing pathogen Gaeumannomyces graminis var. tritici (Ggt) on the seminal and secondary roots of wheat was determined with pot assay in glass house. The result showed that the seminal roots of wheat were highly susceptible to the infection of Ggt. However, the secondary roots were relatively resistant, and the degree of severity was associated with the age of roots and the resistance of wheat cultivars to the take-all disease. In comparison to 76120, 8793-8 was a cultivar resistant to the infection of Ggt.

  Localization and characterization of entFM gene of Bacillus thuringiensis 2009, 25 (19):  213-218.  Abstract + ( 1693 )   PDF ( 893445KB )   ( 819 ) Abstract：The objectives of the study were to determine the location of entFM gene in genome and the characterization of entFM gene sequence. The probe for entFM gene of WB9 was prepared and hybridized with plasmid DNA and chromosomal DNA of WB9 respectively. The result indicated that entFM gene of WB9 is in the chromosome. The ORF of entFM genes in Bt WB9, TS16, 1072 and Bc 6A1 were cloned and sequenced. Sequence analysis showed that entFM gene of WB9 contains 1281 nucleotide acids, encoding 426 amino acids.The predicted amino acid sequences of entFM gene of WB9, TS16, 1072 and 6A1 were compared with Bc Cx5, the result showed that there are high homologous among peptides of WB9,TS16,1072 and Cx5,the main differences between WB9 and Cx5 are that 4 amino acids (Gln-Thr-Gln-Thr) are deleted between 39th and 42th in WB9 and 4 different amino acids(SWDK) are added between 96th and 99th in Bc Cx5.

  RGA Analysis of Near-isogenic Line TcLr38 Resistance to Wheat Leaf Rust 2009, 25 (19):  222-227.  Abstract + ( 1532 )   PDF ( 591262KB )   ( 920 ) Abstract: The aim of the research is to isolate the Resistance gene analogs(RGAs), which will provide solid base for the researches related to Lr38. Degenerate primers designed from conserved NBS-LRR (nucleotide binding site-leucine rich repeat) regions of known disease resistance(R) genes was used to amplify and clone homologous sequences from wheat leaf rust near-isogenic TcLe38. Nine coding sequences named as D-8, A-5, B-20, C-6, F-16, A-4, B-9, C-4 and D-12 were obtained respectively from the RNA of TcLr38. Those RGAs were isolated containing the conserved motifs of NB-ARC, and D-8, A-5, B-20, C-6, A-4, B-9, C-4 and D-12 were deduced corresponding to NBS-containing genes, such as Kinase-2、kianse-3a and hydrophobic domain(HD). Keywords: Wheat leaf rust; TcLr38; NBS-LRR; Resistance gene analog(RGA).

  Studies on the Induce-Resistance Effect of Harpin on Downy Mildew and Botrytis Cinerea of Cucumber 2009, 25 (19):  228-230.  Abstract + ( 1405 )   PDF ( 733491KB )   ( 727 ) Abstract: Harpins are proteins produced by plant pathogenic bacteria; They induce hypersensitive response in non-host plants. The the induce-resistance effect of Harpin was investigated in cucumber. The results show that the 3% HarpinEa 10g-30g + water 30kg / 667 ㎡Were applied 3 times 20 days interval after the cucumber transplanting. The effect of induced resistance on cucumber downy mildew and Botrytis cinerea were respectively up to 58.90% and 44.22%. The effect of different concentrations of HarpinEa had no significant difference. If the occurrence of these two diseases were serious，3% HarpinEa 20g + water 30kg / 667 ㎡combined with the corresponding aerosol released had a better effect and the effect was up to 80.26%.

  Isolation of antifeeding components from Polygonum orientale L. and its effect on digestive enzymes of plutella xylostella larvae 2009, 25 (19):  231-235.  Abstract + ( 1898 )   PDF ( 791106KB )   ( 637 ) For evaluating the insect resistance property of Polygonum orientale L, five extracts of its with petroleum ether, chloroform, ethyl acetate, n-butanol and water were tested for their antifeedant activity against plutella xylostella larvae. The results showed that ethyl acetate extract was the most effective one among the five extracts. In 24h and 48h of treatment, the antifeedant rate of the ethyl acetate extract against 3rd instar larvae of p.xylostella at 5.0mg/mL was 68.42% and 62.55%, respectively. The ethyl acetate extract was further separated through silica gel column and the fractions were monitored with analytical thin layer chromatography (TLC). Thirteen fractions were obtained and also tested for their antifeedant activity against p.xylostella larvae. The bioassay results showed that fraction 8 at 2.5mg/mL had higher antifeedant activity to 3rd instar larvae of p.xylostella than other fractions. The antifeedant rate of fraction 8 was 77.13% in 24h of treatment and 68.53% in 48h of treatment. The inhibiting activity of the 8 fraction against two digestive enzymes glutathion-s-transferase and alkaline phosphatase from the p.xylostella larvae was tested, but carboxylesterase and acid phosphatase were induced.

  Study on Relationship between Resistance to Blast and Yield Traits in Early Japonica Rice in Cold Region 2009, 25 (19):  236-238.  Abstract + ( 1516 )   PDF ( 464190KB )   ( 566 ) Correlation coefficients between rice resistance to blast and yield traits of 316 varieties which were tested in the region trial in the cold regions in recent 5 years. The result showed: A significant negative correlation effect between resistance to leaf and neck blast and growth days, active accumulated temperature in cold region early japonica rice varieties. A significant negative correlation effect between resistance to leaf and neck blast and panicle length, the numbers of panicle per m2 was just the opposite; a significant negative correlation effect between resistance to blast and yield. Show that the rice of growth days longer and active accumulated temperature higher leaf blast and panicle blast incidence of the fewer, more panicle per m2 blast heavier, the stronger the resistance of varieties yield higher in cold areas. So the appropriate choice of reproductive traits of rice varieties, can be selected not only high-yield and disease resistance of new varieties of rice in the cold regions.

  Study on the Optimum Population of Xianyang city Based on Ecological Footprint 2009, 25 (19):  239-243.  Abstract + ( 1402 )   PDF ( 656509KB )   ( 688 ) The ecological optimum population of a district is decided by ecological capacity in the district and district population's need about the ecological resources. This paper, firstly, introduces the key conceptions and procedures of the model, and then calculates the ecological footprint and the utilizable ecological capacity from 1999 to 2006 in Xianyang city. On this basis, the optimum population is estimated from 1999 to 2006 in Xianyang city. During 1999-2001, the actual population of Xianyang city gradually increased from 467.12 ten thousand to 477.32 ten thousand, and the ec-optimum population of Xianyang city gradually increased from 188.86 ten thousand to 195.40 ten thousand. During 2002-2006, the actual population of Xianyang city gradually increased from 481.66 ten thousand to 499.69 ten thousand, and the ec-optimum population of Xianyang city gradually decreased from 177.43 ten thousand to 138.04 ten thousand. To sum up, it revealed that Xianyang city had already in a state of ecological deficit and overpopulation, and the current size of the population was very unreasonable. Finally, the available ways for the sustainable development of Xianyang city in the future were put forward.

  The research progress of the pyroligneons acid applied to agriculture 2009, 25 (19):  244-247.  Abstract + ( 1804 )   PDF ( 483591KB )   ( 1357 ) Abstract:pyroligneous acid is a kind of pure product made from woody material,which possess the merit of simple process,safety,no pollution and remains.There are significance in reality of full utilization of discarded plant resource to developing organic and ecological agriculture,and fullfiling maintainable strategy.in this paper. Besides the introduction of concept about pyroligneous acid,the field of application,the key point is to expounding utilization status of pyroligneous acid in vegetable,crops,edible mushroom,livestock and poultry.,aimed at extending and study of pyroligneous acid in agricultural production.

  The Prevention and Cure to Plant Diseases and Insect Pests and the Hothouse Cherry's Tomato has been Influenced by Low Temperature Cold Disaster Shi Feng-yun 2009, 25 (19):  248-250.  Abstract + ( 1528 )   PDF ( 446098KB )   ( 825 ) Abstract: Cherry's Tomato has like warming and sunlight, the normal growth has been influenced by low temperature cold disaster, could be effect with disease even died. They are hree tandard which has light,middle and power classes,According to the hothouse cherry's tomato has been influenced by different intensities low temperature cold disease , through examines data of Puyang area 1970-2008,conclusion show that : they are 36 times low temperature cold disease course during 39 years, given the changes character of years and emerged frequency of month : and also review the relations between Cherry's tomato and environment, and studying the suitable temperature, sunlight and air humidity, to advance the defence measures and the prevention and cure to plant diseases and insect pests during different growth period to low temperature cold disaster.

  Interspecific Associations Analysis of Principal Species in Natural Vegetation Recovery Shrub Stage in Sloping-land with Purple Soils in Hengyang Basin 2009, 25 (19):  251-256.  Abstract + ( 1361 )   PDF ( 1915611KB )   ( 563 ) Based on 2×2 contingency table,the interspecific association of 20 principal plant species in natural vegetation recovery shrub stage in sloping-land with purple soils in Hengyang Basin was studid.The results showed that:(1)The overall associations among dominant species in plant community had significant negative association;(2) Among 190 pairs of species in higher important values 20 plant populations,58 pairs were significant positive association and 34 pairs were significant were negative association;(3) Based on x2 correction test,together with association coefficient and 3 index in interspecific connection,the interspecific association in 20 principal plant species could be better measured.

  Analysis on the change of thermal resource of winter wheat growth period before over wintering in south Hebei province 2009, 25 (19):  257-262.  Abstract + ( 1409 )   PDF ( 989766KB )   ( 668 ) In the recent 50 years, last day with the temperature ≥0℃, the accumulate temperature of ≥0℃ before Over wintering and monthly mean temperature form October to December changed with big annual fluctuation and the tendency of liner growth of was not obvious .However, In the recent 5 years, last day with the temperature ≥0℃ has been reduced obviously. the accumulate temperature of ≥0℃ before Over wintering increased largely and the monthly mean temperature form October to December was higher. The wavelet analysis indicated last day with the temperature ≥0℃ had mainly the interannual oscillation periods of 4-7 years and 14-17years.and the accumulate temperature of ≥0 before Over wintering had mainly the interannual oscillation periods of 5-10 years and 15-20years. the accumulate temperature of ≥0℃ before Over wintering seems to be increasing for large scale period. Monthly mean temperature form October to December had mainly the interannual oscillation periods of 3-5 years,8-10years and 20-25years and the coming 8-10 years is in the stage that mean temperature would has been higher. The obtained results showed that thermal resource of winter wheat the growth period before over wintering showed an increasing trend and these agrometeorological elements fluctuated in a big way greatly. Therefore, appropriate readjustment should be made on sowings time based on mid long climate prediction avoid affecting on winter wheat growth and development by interannual fluctuation of thermal resource.

  Study on Waterlogging Disaster Index and Risk Assessment Model of Winter Wheat in Jianghuai Region 2009, 25 (19):  263-268.  Abstract + ( 1171 )   PDF ( 1471003KB )   ( 692 )

  The Smart-Growth Theory’s Elicitation to Luan Chuan County’s towns’ Urban Space Expanding and How it is used in Luan Chuan County’s Overall Plan of Land Utilization 2009, 25 (19):  269-273.  Abstract + ( 1528 )   PDF ( 547554KB )   ( 802 ) Abstract: Smart Growth was the most popular conception in the late 1990s, which was suggested by America projector. In a all, the smart growth theory is a compact pattern of city planning, which is going to regulate the city expanding, protect the ecological environment, make good for the develop of the economy, accelerate the pace of harmonious development between the town and the country, and improve the people’s life quality, on the base of increase the utilization efficiency of land. This article is connected the fact of the Luan Chuan’s overall plan of land utilization under the situation that all the cities and towns are making their new land-use planning. Depict the using of the smart growth theory on the Luan Chuan’s city expanding, and how is it be used in the new land-use planning.

  Decolorization of Naphthol Green-B by Removing Nutritional Repression and High Producing Ligninases Mutant of White Rot Fungus pc5305 2009, 25 (19):  274-278.  Abstract + ( 1218 )   PDF ( 690470KB )   ( 619 ) Abstract：Using white-rot fungi to treat dye wastewater can reach a good decolorization purpose by means of their specific degradation enzymes. In this paper, concentration of 300mg/L Naphthol Green-B dyes was used to be treated by white rot fungi. At first, the strain of pc5305 was to be screened for the best decolorization white rot fungus which used to treat Naphthol Green-B from the four different strains. Secondly, we optimized the decolorization conditions of Naphthol Green-B with pc5305. The results showed that the optimal process for pc5305 to decolorize Naphthol Green-B dyes were those of initial pH 4.4,oscillating,using after four days static culture and the inoculation quantity greater than 2.0×107spores/L pc5305 to be inoculated. In this optimal decolorization conditions, the decolorization rate of pc5305 to 300mg/L Naphthol Green-B dyes can reach above 90% within 8h.

  Effect of EDTA and Tartaric Acid on Cu and Pb Releasing from Soil 2009, 25 (19):  283-286.  Abstract + ( 1463 )   PDF ( 585817KB )   ( 699 ) According to study the effect of EDTA, tartaric acid and the associative action of EDTA and tartaric acid on Cu and Pb releasing from soil through batch experiments. The results showed that the releasing amount of Cu and Pb increased with the increase of EDTA or tartaric acid concentration. EDTA promoted more Pb than Cu releasing from soil, while tartaric acid did the reverse. The associative action of EDTA and tartaric acid can notably increase the releasing amount of Cu from soil, the biggest releasing rate was 48%, and it almost released the same amount of Pb with EDTA in soil alone.

  Variations of Soil Mineral Elements in reversion process of desertification in arid areas 2009, 25 (19):  287-290.  Abstract + ( 1367 )   PDF ( 533913KB )   ( 680 ) At the South Edge of the Tengger Desert，the sampled sites of mobile sanddune, semi-fixed sanddune and fixed sanddune were selected as the different stages of the reversion process of desertification with the method of substituting time with space, and topsoil mineral elements of the decertified land were studied in reversion process of desertification. The results showed that the content of S, P, Br, Cl, Sc, Nd, La, Ga, Rb and Sn of topsoil increased, the content of Cu, Mn, Zn, Ni, Nb, Cr, Pb, V, Bi, Hf, As, Y, Ce, Th, Sr, Zr, Ti, Mg, Ca, Na and Fe of topsoil reduced first and increased later, and the content of W, Co, Cs, Tl, U and K of topsoil increased first and reduced later while the content of Ba and Si of topsoil reduced in reversion process of desertification. Obviously, with the reversion process of desertification, sand-binding vegetation recovered, soil structure improved and soil fertility increased, and the contents of topsoil mineral elements changed distinctly.

  The Progress of Study on Soil Improvement Research with Straw Stalk 2009, 25 (19):  291-294.  Abstract + ( 1541 )   PDF ( 516556KB )   ( 1682 ) Crop stalks are a resource utilization of solid waste, which contain a large number of carbon, nitrogen, phosphorus, and other nutrients. At the same time, they are rich in cellulose, hemicelluloses, and a certain amount of lignin, protein and sugar and other organic substances. Stalks can improve the structure of soil and keep the ability of water, fertilizer, gas and heat, decrease soil bulk density, increase porosity, improve the content of organic matter, make nutritional structure tend to reasonable. Straw returning can save the amount of chemical fertilizers, reduce production costs. It also can reduce the environmental pollution, ensure the agricultural production in virtuous cycle, protect rural ecological environment, promote the development of ecological agriculture.

  Comparison of the coumarin content of roots in the different years of Glehnia littoralis XIN Hua 2009, 25 (19):  295-297.  Abstract + ( 1370 )   PDF ( 487083KB )   ( 676 ) The content of some coumarins in roots of Glehnia littoralis were determined and compared with HPLC. The study shows that the larger the number of grown years, the more the content of psoralen, imperatorin，isoimperatorin and the gross amount of the three. The coumarin content in the roots of Glehnia littoralis of four grown years is extremely significantly more than the other ones. Therefore, roots of Glehnia littoralis of two or more grown years should not be kept only for seeds in production, and also, they should be used as medicinal material.

  Some Physiological Changes in Chamaedorea elegans Seedling under Low Temperature Stress 2009, 25 (19):  298-302.  Abstract + ( 1793 )   PDF ( 746057KB )   ( 719 ) Abstract:Under 2℃ low temperature stress , malondialdehyde (MDA) content in leaves of Chamaedorea elegans H. Wendl. seedling gradually increased, reflecting that membrane lipid peroxidation aggravated. Electrolyte leakage increased to 92.35% after 3d, cell membrane was damaged irreversible. Cell defense enzymes SOD, POD and CAT activity raised at early stage, and then lowered, especially enzymes CAT falls greatly. Net photosynthetic rate in leaves decreased to -1.89 μmol CO2 ?cm-2?s-1 after 5d； Transpiration rate in leaves changed little in 4d, but sharp declined after 5d.

  Construction of Fujian Agriculture Sci-tech integrated information management system 2009, 25 (19):  303-308.  Abstract + ( 1500 )   PDF ( 798327KB )   ( 714 ) Abstract: This paper analyzed the problems of Agriculture Sci-tech informatization; put forward the solutions for informatization construction; developed an agriculture information-based applied management system, which involves digital collection and storage, digital network, information analysis and treatment, and digital control management; and discussed the general ideas of this research., main research topics, the construction of system platform, testing operation performance and application prospect.

  Optimization for enzymolysis of straw under the condition of microwave pretreatment and ultrasound-assisted 2009, 25 (19):  314-318.  Abstract + ( 1606 )   PDF ( 1615896KB )   ( 819 ) To improve the efficiency of enzymolysis, soybean straw was pretreated by using the microwave reactor and enzyme hydrolyzed by ultrasound-assisted method. By means of the scanning electron microscopy (SEM) analysis, the compact structure of soybean straw is evidently destructed after the microwave pretreatment, and was beneficial to hydrolyze by cellulase. Condition of microwave pretreatment is optimized by orthogonal experimental. As result, the optimal conditions for the microwave pretreatment of soybean straw are shown as follows: microwave irradiation power 400W, irradiation time 40 min, irradiation temperature 60 ℃. In optimized condition of the microwave pretreatment and ultrasound-assisted enzymolysis, the enzymatic hydrolysis yield of the straw is 11.06% at 7 h, which was almost equal as the yield (11.77%) of the conventional enzymolysis at 48 h. The enzyme efficiency increased significantly.

  The Dynamic Variation of soil temperature on the Dryland winter Wheat under Various mulching Models 2009, 25 (19):  319-322.  Abstract + ( 1201 )   PDF ( 488241KB )   ( 609 ) Abstract: Soil temperature date under different mulching modules and different depth and different growth stage of winter wheat in dryland was analyzed. The results showed that overlaying cultivation can't change the variety regulation of soil temperature. Film mulching and hole sowing cultivation remarkably increase 0～25cm soil temperatures before green-turning stage, Furrow-film mulching cultivation effective increase surface layer soil temperatures before winterovering stage, Straw mulching cultivation has effect of increasing soil temperature in seedling stage, and decreasing soil temperature after green-turning stage.

  Relationship Between Distribution of Meiofauna and Environmental Factors in Southern Yellow Sea 2009, 25 (19):  323-329.  Abstract + ( 1429 )   PDF ( 797722KB )   ( 819 ) The abundance and biomass of meiofauna were quantitatively studied at a grid of 28 stations during the cruise of investigation in the Southern Yellow Sea, China in January 2004. The main results are as follows: A total of 20 groups of meiofauna in the Southern Yellow Sea were identified, they were Nematoda, Copepoda, Polychaeta, Kinorhyncha, Bivalvia, Ostracoda et al.. The mean abundance and biomass of meiofauna are (1186.12 ±486.07) ind/10cm2 and (1120.72 ±487.21) μg/10cm2, respectively. Free living marine nematodes are the most dominant group, accounting for 89.702% of the abundance of meiofauna, with benthic harpacticoid copepods in the second, accounting for 4.193%. In terms of horizontal distribution, stations near the coast have higher abundance and biomss of meiofauna. In terms of vertical distribution, 74.33% of total meibenthos is found in the surface sediment 0-2cm, 22.31% in 2-5cm and 3.36% in 5-8cm sediment. Correlation analyses between abundance and biomass of meiofauna and environmental factors show that Chl-a is the key factor that affects the distribution of meiofauna.

  The Countermeasure And The Tourism Decision-Making Behavior Characteristics Of Tourists From Leisure Agriculture In Small And Medium-Sized Cities ——Take the Jiulonggu and happy farm scenic of leisure agriculture in Putian City in Fujian Province as an example 2009, 25 (19):  330-335.  Abstract + ( 1434 )   PDF ( 649089KB )   ( 756 ) The article take the Jiulonggu and happy farm scenic of leisure agriculture in Putian City in Fujian Province as the recreation area for study. It use the interviews and field survey methods to analyze the demographic characteristics of tourists samples ,and discuss and summary the tourism decision-making behavior characteristics of tourists from leisure agriculture in small and medium-sized cities through the tourism motivation, the load of access to tourism information, the decision-making to the tourism purpose, the tourism consumption, way out, the most favorite tourism activities Etc.. Finaly it put forward to some strategies to improve and enhance the tourism development of leisure agriculture in the small and medium-sized urban.

  Research on Agricultural Land Transfer in Balancing Urban and Rural Development Pilot Area ——A Case Study of Beibei District of Chongqing Municipality 2009, 25 (19):  336-340.  Abstract + ( 1606 )   PDF ( 571621KB )   ( 773 ) Using a large data set gathered in household surveys conducted in Beibei District of Chongqing Municipality, Analysis of agricultural land transfer in the existing status quo in Beibei District, summed up the agricultural land transfer at this stage of the characteristics and problem. The results show: Uneven distribution of agricultural land in the Transfer and a lower turnover rate, Transfer of agricultural land market development slow, Agricultural land irregular flow management, Ural social security have imperfect. On this basis, put forward the proposal of improve Transfer of agricultural land market, Improve the rural social security, Standardize the transfer of agricultural land management, In order to helping local government departments to develop policies to provide practical and scientific basis for decision-making.

  On the Resolve Way of the Disputes of Land in China＇s Transitional Period：Interpretation of Current Situation and Legal Regulation 2009, 25 (19):  341-344.  Abstract + ( 1186 )   Abstract:In China’s transitional period，the cases of disputes of land are shown a growing number of cases and often in situations for the conclusion of the cases, which are very damaging to the construction of a harmonious society. In order to cope with such challenges and achieve effective maintenance and efficient use of land resources, this papers will go to find the corresponding answer and the way of corresponding legal regulation of disputes of land，on the basis of interpretation of current situation and summarization of characteristics of the disputes of land.

  Study on the Management and Evaluation Index of the Development of the Characteristic Crop Farming Science and Technology Park 2009, 25 (19):  345-348.  Abstract + ( 1390 )   PDF ( 442341KB )   ( 747 ) This article has been selected agricultural science and technology parks play an important role in farming science and technology parks for research, and Zhongning wolfberry in the production of industrial standardization GAP demonstration garden in Ningxia as a typical case in an attempt to establish a scientific and effective for its comprehensive evaluation index system.

  The Trouble Shooting of Shaanxi Northern Apple Industry Groups across Global Financial Crisis 2009, 25 (19):  349-352.  Abstract + ( 1290 )   PDF ( 503578KB )   ( 792 ) A deep analyzing and re-orienting is based on “diamond theory” for Shannxi Northern Apple Industry Groups, in order to find out the answer about the global financial crisis. It is improved the competitive advantages by replace desalting and recessionary factors to new standing development dynamic factors via the innovative actives of the industry groups. Though assorting with interior functions involution, keep ahead to healthy develop the Shannxi Northern Apple Industry Groups.

  China’s countryside impoverished government present situation and the practice way have a limited view of LI Wen_Zheng 2009, 25 (19):  353-356.  Abstract + ( 1181 )   PDF ( 469157KB )   ( 618 ) China’s countryside impoverished question still exists， and becomes the new rural reconstruction a big barrier.Presently， our country countryside impoverished government has the relatively impoverished problem to be prominent， the impoverished population returns poorly rate is high， actual impoverished population quantity is big and so on the questions. Therefore，Must develop the rural education vigorously, the reform lateral economic ties, the break dual society structure, the construction transmission mechanism, the perfect agricultural finance legislation, enlarges to the agricultural countryside farmer's fund support dynamics, the land right of disposal rise for the soil capital function, the display poor area surplus-labor superiority, consummates the surveillance mechanism.