Chinese Agricultural Science Bulletin ›› 2009, Vol. 25 ›› Issue (19): 37-41.
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Abstract：The factors influencing ISSR-PCR to analyze the genetic divergence in Wampee were investigated by a single factor test and orthogonal design experiment.The results showed that a better amplification system of ISSR was obtained with the reation system comtaining 30ng template DNA,1.5U Taq DNA polymerase,0.4μmol/L primers,0.3mmol/L dNTPs in the total volume of 20μL.The optimized annealing temperature was 52.4℃ for primer (gA)8C.
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URL: https://www.casb.org.cn/EN/
https://www.casb.org.cn/EN/Y2009/V25/I19/37
