Abstract:

Five methods namely improved CTAB method, CTAB-silica extraction method, SDS method, low pH extraction method with high salt and Zhou shiliang method were adopted to extract genome DNA from the leaves of Citrus aurantium. And three methods including ultraviolet spectrophotometer, agarose gel electrophoresis and SSR-PCR amplification were used to detect extracted sample DNA. The results indicate that SDS method is better than other 4 DNA extraction methods, DNA concentration is highest（Its average is 900 ng/ul）and DNA purity is best（Its average is 1.90）, and SSR-PCR amplification results with UV-spectrophotometer and gel electrophoresis test results are basically the same. So SDS method is the best DNA extraction method for Citrus aurantium, and DNA quality suitable for molecular detection experiment.