Abstract:

Abstract: The aim of the research is to isolate the Resistance gene analogs(RGAs), which will provide solid base for the researches related to Lr38. Degenerate primers designed from conserved NBS-LRR (nucleotide binding site-leucine rich repeat) regions of known disease resistance(R) genes was used to amplify and clone homologous sequences from wheat leaf rust near-isogenic TcLe38. Nine coding sequences named as D-8, A-5, B-20, C-6, F-16, A-4, B-9, C-4 and D-12 were obtained respectively from the RNA of TcLr38. Those RGAs were isolated containing the conserved motifs of NB-ARC, and D-8, A-5, B-20, C-6, A-4, B-9, C-4 and D-12 were deduced corresponding to NBS-containing genes, such as Kinase-2、kianse-3a and hydrophobic domain(HD). Keywords: Wheat leaf rust; TcLr38; NBS-LRR; Resistance gene analog(RGA).