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Chinese Agricultural Science Bulletin ›› 2009, Vol. 25 ›› Issue (22): 0-0.

   

Construction of an highly producing dsRNA Escherichia coli strain BL21 RNase Ⅲ-

  

  • Received:2009-07-01 Revised:2009-07-20 Online:2009-11-20 Published:2009-11-20

Abstract: RNase Ⅲ was deleted in Escherichia coli BL21 chromosome using homologous recombination system PKD46. A RNase Ⅲ- recombinant bacterium strain that can highly producing dsRNA was obtained. The recombinant bacterium strain would be able to lay foundation for applications of RNAi cheaply.