Abstract:

Phalaenopsis amabilis was cultured in the experiment. Its tissue culture system ,especially on the optimum formula of culture medium and culture procedure at each phase was researched by using tender peduncles before flowering period, peduncles after flowering period and shoot tip as explants. The results showed : peduncles after flowering period were fit for inducing germination of axillary buds. In MS medium with BA1mg/L and NAA0.1 mg/L, the axillary buds of peduncles could be induced to germinate easily. The best way in prevention shoot tip from browning is cutting the shoot tip in a sterile petri dish with a filter-sterilized solution of 1%VC to the medium with 2%PVP.In the VW medium with BA3mg/L , NAA0.5 mg/L and AC 200ml/L, shoot tip tended to be induced to germinate buds. The best differentiation and proliferation medium is MS+ BA1mg/L+NAA1 mg/L. The rooting medium is ?MS +IBA1mg/L+NAA1 mg/L. After training , the cultured plantlets were transplanted in wet algae ,and keep the humidity above 80%.