Abstract:

The relationship and genetic diversity among 7 Arachis germplasm which are resistant to bacterial wilt from ICRISAT mini core collection and China core collection were systematically investigated based on SSR markers and botanic traits. Resistance of two genotypes acquired from 155 accessions of ICRISAT mini core collection to bacterial wilt is as high as 91.7%. Twenty-four SSR primer pairs with polymorphic bands ranging from 2 to 7 were selected through 58 primer pairs screening. The genetic distance varied from 0.17~0.71 with average of 0.49 among Arachis species. The distance between the accession ICG12625 and two genotypes(Zuodou、Fuchuan) showed the largest(0.71),while the least distance was detected between Shengxian and Fuchuan. There were 12 pairs of accession of which distance was larger than 0.50. The DNA molecular characters and fingerprints of the 7 accessions were detected based on SSR products.Clustering result had showed that ICG12625 had larger distance than other genotypes and was divided into a subspecies alone. ICG12625 could be used as breeding genotypes.