Abstract:

In order to rapidly and economically obtain high-quality sesame genomic DNA for molecular experiments, an efficient method of DNA extraction suitable for sesame was explored, referred methods in other crops, the procedure was simplified and the cost was reduced, DNA were detected by agarose electrophoresis and no trailing phenomenon was found, their OD260/OD280 values were all between 1.8-2.0, indicating that qualities of DNA were good and high purity. Afterwards, in order to further improve the efficiency of molecular marker experiments, we established a rapid and effective silver staining procedure for polyacrylamide gels, by improving the traditional silver staining methods and controlling the staining and imaging time.