Abstract:

Molecular marker technology provides a powerful tool for understanding the genetic differences between individuals from the DNA level directly.It is an important direction to analyse the correlation between parental genetic distance and the growth traits of their offspring with the DNA molecular markers. Eight Japanese flounders(JS) and 24 resistant flounders(RS) were used to make 28 crosses to analyse the genetic diversity of 32 parents,using 21 polymorphic microsatellite markers.Simultaneously, the correlation between parent SSR genetic distance and the growth rate of their offspring was assayed.The results indicated that:(1) the number of total alleles of these 21 microsatellite loci was 123，the allele number (A)，effective allele number (Ae)，observed heterozygosity (Ho)，expected heterozygosity (He) and polymorphism information content（PIC），ranged from 2~15，1.3423~7.8168，0.2333~1.000，0.2593~0.8859，0.2225~0.8613，respectively.The average A，Ae，Ho，He，PIC was 5.86, 3.7951,0.6931,0.6784,0.6237，respectively. (2)The minimum genetic distance was 0.2578,while the maximum was 0.9773.The correlation between AGRw of offspring and parental GD existed considerable difference in different range of genetic distance. As the GD for 0.2578~0.5958, the correlation between AGRw of offspring and parental GD appeared to be positively significant at P<0.05；As the GD for 0.6099~0.6604，there was no significant correlation；Furthermore,as the GD for 0.6640~0.9773,the correlation between AGRw of offspring and parental GD appeared to be negatively significant at P<0.05.