Abstract:

In order to obtain better quality total RNA in different parts of peanuts, two RNA extraction methods (CTAB and Trizol) and two RNA extraction kits (AXYGEN and TIANDZ) were compared. The results showed that the improved CTAB method was better than Trizol method and two total RNA extraction kits. The total RNA isolated with the improved CTAB method had high integrity, high yield, good stability and no DNA pollution, and then could be used for semi-quantitative RT-PCR and so on.