Abstract:

In this experiment, segregating from an F1 contains 240 separate population of an intraspecific crossed between ‘Ku50’, as female parent, and ‘SC124’ as male parent. We obtained 269 polymorphic markers when used 110 polymorphic primer pairs with the F1 populations, and basing on these 269 polymorphic markers, a genetic linkage map that consisting of 140 molecular markers, including 111 expressed sequence tag (EST)-SSRs, twenty-two simple sequence repeats (SSRs) and 7 sequence-related amplified polymorphisms (SRAPs) was constructed by using Joinmap3.0 software, The genetic map contained 21 linkage groups, and LG1 and LG3 had the most markers, holding 15 markers, however the least linkage group LG21 only had two markers. The genetic map spanned a 1341.775 cM genetic distance, with the longest linkage group LG3 was 132.904 cM, as well as the smallest LG19 was 0.431 cM, and the average marker interval was 9.391 cM.