Abstract:

Blast is the most serious rice diseases in the world. The prevention and controlling of it is therefore significant to the agriculture production. With the rapid development of bio-technology, an in vitro screening technique system in tissue culture for rice (Oryza sativa L. subsp. indica) was widely applied as an effective method to the resistant breeding of rice. We review three main aspects of this technique: the callus induction, the preparation of crude toxin factor and the selection of toxin resistant materials. Furthermore, we introduce questions and prospects of the application of these mutants in rice resistant breeding.