Abstract:

To construct the expression vector with the candidate genes of bacteria blight disease resistance gene Xa31(t) in rice, and to make the genetic transformation, the candidate genes Xa31(t)-1 and Xa31(t)-2 of rice bacterial blight resistance gene Xa31(t) were obtained through the method of long segments PCR with the resistant variety ‘Zachanglong’ (ZCL) genomic DNA as the template. Then the target genes were cloned to the T-vector with the way of restriction endonuclease reaction and enzyme ligation reaction. After screening the positive clones and sequencing, the target genes were cloned to the expression vector of pCMABIA1300. Finally, the recombinant plasmids were transformed into the callus of the susceptible varieties ‘Nipponbare’ and ‘Taibei309’ respectively by agrobacterium-mediated approach and a lot of transformed rice seedlings were obtained. These work laid the foundation for cloning the bacterial blight resistance gene Xa31(t) and studied its function.