Abstract:

The plant sweet protein MabinlinⅡ is found rich in mature seeds of Capparis masaikai Lévl which is particular to China and may have wide application prospects in food sweeteners industry. In this study, in order to provide a reliable testing antibody for engineering applications of recombinant MabinlinⅡ, MabinlinⅡ was separated and purified from the mature seeds of Capparis masaikai Lévl by ion exchange chromatography. The purified protein was used as antigen to immunize New Zealand rabbits to prepare polyclonal antibody against the MabinlinⅡ protein. The result of Enzyme-Linked Immunosorbnent Assay (ELISA) showed that the titer of the polyclonal antibody to purified MabinlinⅡ was 1:256000, and the polyclonal antibody against the purified MabinlinⅡ also had highly reactivity and specialty in western blot analysis. The polyclonal antibody against MabinlinⅡ could be used for detecting the protein amounts of MabinlinⅡ expressed in various bioreactors, which may serve sensitive and reliable methods for immunology detection in genetic engineering research of MabinlinⅡ.