Abstract:

In order to understanding the dynamic change of microbial communities during composting process, this research summarized molecular biotechnologies widely used in composting, including principles, methods and skills, problems and countermeasures during the application of DGGE/TGGE, SSCP, T-RFLP, ARDRA/RFLP, RAPD technology. In conclusion, we should made full use of traditional culture and molecular biology techniques to discuss succession rules of important flora during composting. The objective of this study was to comprehensive understanding nature of composting process, and providing scientific basis and theoretical guidance for the research of compost technology and process.