Abstract:

To study the separation and purification of cellulase from crude enzyme, the method of ammonium sulfate fractionation, Sephadex G-100 gel filtration and DEAF FF weak anion exchange column chromatography method were used. The results showed that: the usage of ammonium sulfate fractionation, Sephadex G-100 gel column chromatography, DEAF FF weak anion exchange column chromatography is a efficient method to purify CMCase. The specific activity of CMCase increase 5.89 times purifed in this way, while the recovery rate was 22%.